Abstract
A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-β-d-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J’sphere ODS C18 column (250 × 4.6 mm, 4 µm). The elution was done using water with 0.1 % phosphoric acid (A) and acetonitrile with 0.1 % phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r 2 > 0.999) within the test ranges. This method showed good recovery and reproducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53 % (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28 % for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex.
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Acknowledgments
This work was supported by a Grant from the National Center for Standardization of Herbal Medicine funded by the Ministry of Food and Drug Safety, Republic of Korea (12172MFDS890).
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Zhao, B.T., Jeong, S.Y., Kim, T.I. et al. Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV. Arch. Pharm. Res. 38, 2183–2192 (2015). https://doi.org/10.1007/s12272-015-0642-3
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DOI: https://doi.org/10.1007/s12272-015-0642-3