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Yin-Yang 1 and Yin-Yang 2 exert opposing effects on the promoter activity of interleukin 4

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Abstract

Interleukin (IL)-4 acts on T cells as a growth and activation factor, and promotes the differentiation of type 2 T helper cells. In T cells, expression of the gene encoding IL-4 is regulated by inducible or constitutive factors. Yin-Yang (YY)-1 is one of constitutive transcription factors binding to the IL-4 promoter. The recently identified YY2 protein is similar to YY1, with both sharing high levels of homology in their zinc finger motifs. However, the role of YY2 in T cells is unclear. YY1 and YY2 were constitutively expressed in EL4 T cells, and their expression was not dependent on stimulation. IL-4 promoter (−741/+56 fragment) activity was enhanced by YY1, but inhibited by YY2. The enhanced IL-4 promoter activity by YY1 was reduced by simultaneous expression of YY2. In addition, the DNA binding affinity of YY1 to the IL-4 promoter was adversely affected by YY2. Our results suggest that YY1 and YY2 exert opposing effects on the IL-4 promoter as they compete for the same DNA binding sites.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0006261).

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Authors and Affiliations

  1. College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju, Republic of Korea

    Sung Ho Lee, Hyung Min Jeong, Kwang Youl Lee & Bok Yun Kang

  2. College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea

    Young-Chang Cho

  3. Department of Food and Nutrition, Mokpo National University, Muan, Jeonnam, Republic of Korea

    Kyung Hee Kim

  4. College of Pharmacy, CHA University, Seongnam, Gyeonggi, Republic of Korea

    Hyun Jin Choi

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  1. Sung Ho Lee
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Lee, S.H., Cho, YC., Jeong, H.M. et al. Yin-Yang 1 and Yin-Yang 2 exert opposing effects on the promoter activity of interleukin 4. Arch. Pharm. Res. 39, 547–554 (2016). https://doi.org/10.1007/s12272-015-0622-7

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  • DOI: https://doi.org/10.1007/s12272-015-0622-7

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