Abstract
In this study, we attempted to determine the anti-inflammatory activity of a medicinal plant huang-lian using gene expression profiles as an index. Huang-line extracts (CEXs) were prepared from seven different plant origins and compared for their chemical composition and biological activity. In order to achieve this, RAW264.7 cells were treated with CEXs in the absence or presence of LPS for 6 h, and the differential gene expression profiles were analyzed using oligonucleotide microarrays. The alkaloid content of CEXs was determined by high performance liquid chromatography analysis. Evaluation of anti-inflammatory activity of CEXs was by measuring a decrease in cytokines and nitric oxide production in LPS-stimulated RAW264.7 cells. Hierarchical clustering analysis revealed that three CEXs from Coptis chinensis formed a cluster separate from the other four CEXs in LPS-stimulated cells, and were the most effective anti-inflammatoryagents. The extract prepared from Picrorrhiza kurrooa neither induced any changes in gene expression profiles nor possessed any anti-inflammatory activity. The extract from Jeffersonia dubia, which exhibited the highest cytotoxicity among the CEXs tested, was most effective in suppressing LPS-induced nitric oxide production but was not able to inhibit proinflammatory cytokine production. The results obtained in this study demonstrate that overall gene expression profiles of the extracts correlated well with their biological activity and that CEXs prepared from plants of diverse origins vary in their biological activity. These data also suggest that gene expression profiles may serve as a good indicator for the pharmacological activities of medicinal plants arising from diverse origins.
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Cho, J. Y., Park, J. S., Yoo, E. S., Yoshikawa, K., Baik, K. U., Lee, J. S., and Park, M. H., Inhibitory effect of lignans from the rhizomes of Coptis japonica var. dissecta on tumor necrosis factor-alpha production in lipopolysaccharide-stimulated RAW264.7 cells. Arch. Pharm. Res., 21, 12–16 (1998).
Cho, J. Y., Park, J. S., Kim, P. S., Chae, S. H., Yoo, E. S., Baik, K. U., Lee, J. S., and Park, M. H., Inhibitory effect of oriental herbal medicines on tumor necrosis factor-α production in lipopolysaccharide-stimulated RAW264.7 cells. Nat. Prod. Sci., 5, 12–19 (1999).
Cho, J. Y., Baik, K. U., Yoo, E. S., Yoshikawa, K., and Park, M. H., In vitro antiinflammatory effects of neolignan woorenosides from the rhizomes of Coptis japonica. J. Nat. Prod., 63, 1205–1209 (2000).
Cho, J. Y., Kim, A. R., and Park, M. H., Lignans from the rhizomes of Coptis japonica differentially act as antiinflammatory principles. Planta Med., 67, 312–316 (2001).
Choi, B. H., Ahn, I. S., Kim, Y. H., Park, J. W., Lee, S. Y., Hyun, C. K., and Do, M. S., Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp. Mol. Med., 38, 599–605 (2006).
Chung, I. M. and Paik, S. B., Isolation and activity test of antifungal substance from Coptidis Rhizoma. Kor. Soc. Anal. Sci., 10, 153–159 (1997).
Cuellar, M. J., Giner, R. M., Recio, M. C., Manez, S., and Rios, J. L., Topical anti-inflammatory activity of some Asian medicinal plants used in dermatological disorders. Fitoterapia, 72, 221–229 (2001).
Dinarello, C. A., The biological properties of interleukin-1. Eur. Cytokine Netw., 5, 517–531 (1994).
Frosch, M., Metze, D., Foell, D., Vogl, T., Sorg, C., Sunderkotter, C., and Roth, J., Early activation of cutaneous vessels and epithelial cells is characteristic of acute systemic onset juvenile idiopathic arthritis. Exp. Dermatol., 14, 259–265 (2005).
Hamilton, J. A., Colony-stimulating factors in inflammation and autoimmunity. Nat. Rev. Immunol., 8, 533–544 (2008).
Hata, H., Sakaguchi, N., Yoshitomi, H., Iwakura, Y., Sekikawa, K., Azuma, Y., Kanai, C., Moriizumi, E., Nomura, T., Nakamura, T., and Sakaguchi, S., Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J. Clin. Invest., 114, 582–588 (2004).
Hu, J. P., Takahashi, N., and Yamada, T., Coptidis rhizome inhibits growth and protease of oral bacteria. Oral Dis., 6, 297–302 (2000).
Kang, J. X., Liu, J., Wang, J., He, C., and Li, F. P., The extract of huanglian, a medicinal herb, induces cell growth arrest and apoptosis by upregulation of interferon-beta and TNF-alpha in human breast cancer cells. Carcinogenesis, 26, 1934–1939 (2005).
Min, B. S. and Cho, J. S., Quantitative determination of protoberebrines from the roots of Coptis chinensis. Nat. Prod. Sci., 14, 68–72 (2008).
Mohamed-Ali, V., Flower, L., Sethi, J., Hotamisligil, G., Gray, R., Humphries, S. E., York, D. A., and Pinkney, J., β-adrenergic regulation of IL-6 release from adipose tissue: in vivo and in vitro studies. J. Clin. Endocrinol. Metab., 86, 5864–5869 (2001).
Niki, Y., Yamada, H., Seki, S., Kikuchi, T., Takaishi, H., Toyama, Y., Fujikawa, K., and Tada, N., Macrophage- and neutrophil-dominant arthritis in human IL-1 alpha transgenic mice. J. Clin. Invest., 107, 1127–1135 (2001).
Ohshima, S., Saeki, Y., Mima, T., Sasai, M., Nishioka, K., Nomura, S., Kopf, M., Katada, Y., Tanaka, T., Suemura, M., and Kishimoto, T., Interleukin 6 plays a key role in the development of antigen-induced arthritis. Proc. Natl. Acad. Sci. U.S.A., 95, 8222–8226 (1998).
Park, H. J., Kim, Y. J., Leem, K., Park, S. J, Seo, J. C., Kim, H. K., and Chung, J. H., Coptis japonica root extract induces apoptosis through caspase 3 activation in SNU-668 human gastric cancer cells. Phytother. Res., 19, 189–192 (2005).
Sansom, D. M., Manzotti, C. N., and Zheng, Y., What’s the difference between CD80 and CD86? Trends Immunol., 24, 313–319 (2003).
Sasai, M., Saeki, Y., Ohshima, S., Nishioka, K., Mima, T., Tanaka, T., Katada, Y., Yoshizaki, K., Suemura, M., and Kishimoto, T., Delayed onset and reduced severity of collagen-induced arthritis in interleukin-6-deficient mice. Arthritis Rheum., 42, 1635–1643 (1999).
Sun, J., Ma, J. S., Jin, J., Wang, H. S., Wen, Q. H., Zhang, H. G., and Zhou, Q. L., Qualitative and quantitative determination of the main components of huanglianjiedu decoction by HPLC-UV/MS. Yao Xue Xue Bao, 41, 380–384 (2006).
Tanabe, H., Suzuki, H., Mizukami, H., and Inoue, M., Double blockade of cell cycle progression by coptisine in vascular smooth muscle cells. Biochem. Pharmacol., 70, 1176–1184 (2005).
Yang, J., Wang, H. D., Lu, D. X., Wang, Y. P., Qi, R. B., Li, J., Li, F., and Li, C. J., Effects of neutral sulfate berberine on LPS-induced cardiomyocyte TNF-alpha secretion, abnormal calcium cycling, and cardiac dysfunction in rats. Acta Pharmacol. Sin., 27, 173–178 (2006).
Yu, H. H., Kim, K. J., Cha, J. D., Kim, H. K., Lee, Y. E., Choi, N. Y., and You, Y. O., Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J. Med. Food, 8, 454–461 (2005).
Zenz, R., Eferl, R., Kenner, L., Florin, L., Hummerich, L., Mehic, D., Scheuch, H., Angel, P., Tschachler, E., and Wagner, E. F. Psoriasis like skin disease and arthritis caused by inducible epidermal deletion of Jun proteins. Nature, 437, 369–375 (2005).
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Kim, J.M., Jung, H.A., Choi, J.S. et al. Comparative analysis of the anti-inflammatory activity of Huang-lian extracts in lipopolysaccharide-stimulated RAW264.7 murine macrophage-like cells using oligonucleotide microarrays. Arch. Pharm. Res. 33, 1149–1157 (2010). https://doi.org/10.1007/s12272-010-0803-3
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DOI: https://doi.org/10.1007/s12272-010-0803-3