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The mechanism of the late preconditioning effect of 3-nitropropionic acid

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Abstract

The aim of the present study was to investigate the mechanism of effect of 3-nitropropionic acid-(3-NP) induced late preconditioning in rat heart. For this purpose 20–30 min before 3-NP (20 mg/kg, i.p.) injection, the rats were treated intraperitoneally with 5-hydroxydecanoate (40 mg/kg, 5-HD, mitochondrial KATP-channel blocker), L-NAME (100 mg/kg, NOS inhibitor), N-2-mercaptopropionylglycine (100 mg/kg, MPG, free radical scavenger), or superoxide dismutase+catalase (10000+10000 IU/kg, SOD+CAT). Control rats received saline only without 3-NP pretreatment. After two days, hearts were isolated and perfused at a constant pressure in a Langendorff apparatus. 15-min global ischemia followed by 30-min reperfusion was applied to all hearts. Pretreatment of 3-NP significantly reduced infarct size, creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) levels, and incidence of ventricular tachycardia (VT) compared with the control group receiving saline only. 5-HD, L-NAME, MPG, or SOD+CAT treatment statistically reversed 3-NP-induced reduction in infarct size. Although CK-MB, LDH levels, and incidence of VT were also reduced by L-NAME, MPG, or SOD+CAT treatment, only 5-HD significantly inhibited beneficial effects of 3-NP on all of the parameters above. These results showed that mito-KATP channels play a pivotal role in late preconditioning effect of 3-NP in the isolated rat heart. However, other mediators such as reactive oxygen species and NO may be, at least in part, involved in mechanisms of this effect.

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Authors and Affiliations

  1. Faculty of Medicine, Department of Medicinal Biochemistry, Gazi University, Besevler, Ankara, Turkey

    K. Okhan Akın & Nedret Kılıc

  2. Faculty of Pharmacy, Department of Pharmacology, Yeditepe University, Icerenkoy, Istanbul, Turkey

    Iclal Cakıcı

  3. Faculty of Pharmacy, Department of Pharmacology, Gazi University, Etiler, Ankara, Turkey

    Bilgen Basgut, Eda Aypar, Ertug Basgut, K. Okhan Akın, Nedret Kılıc & Iclal Cakıcı

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  1. Bilgen Basgut
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  2. Eda Aypar
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Correspondence to Bilgen Basgut.

Additional information

Preliminary findings of the present study were presented at the 17th Annual Meeting of the Mediterranean Association of Cardiology and Cardiovascular Surgery (September 22–24, 2005, Portorož, Slovenia).

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Basgut, B., Aypar, E., Basgut, E. et al. The mechanism of the late preconditioning effect of 3-nitropropionic acid. Arch. Pharm. Res. 31, 1257–1263 (2008). https://doi.org/10.1007/s12272-001-2104-3

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  • DOI: https://doi.org/10.1007/s12272-001-2104-3

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