Abstract
Atomic structures of large biological molecules were first established by scattering X-rays in protein crystals and later with crystals of nucleic acids. Good crystals allow for an accuracy of 0.1 Å (10−11 m) that may reveal details of catalytic processes. The novel cryo-electronmicroscopy method does not need crystals, it can establish chain folds confidently. Chain folds can also be derived from NMR data producing numerous binary atomic distances. Recently, chain folds for a given amino acid sequence were derived by mere computing, based on the large fundus of structurally related proteins.
Literatur
Hünefeld FL (1840) Der Chemismus in der thierischen Organisation. Brockhaus, Leipzig, 158–163
Hoppe-Seyler F (1862) Über das Verhalten des Blutfarbstoffs im Spektrum des Sonnenlichtes. Virchows Archiv 23: 446–449
Miescher JF (1871) De Über die chemische Zusammensetzung der Eiterzellen. Medicinisch-chemische Untersuchungen (ed. Hoppe-Seyler) 4: 441–460
Vonrhein C, Schlauderer GJ, Schulz GE (1995) Movie of the structural changes during a catalytic cycle of nucleoside monophosphate kinases. Structure 3: 483–490
Astbury WT, Street A (1931) X-Ray Studies of the Structure of Hair, Wool, and Related Fibers. Phil Trans Roy Soc London A 230: 75–101
Bernal JD, Crowfoot D (1934) X-Ray Photographs of Crystalline Pepsin. Nature 133: 794–795
Bokhoven C, Schoone JC, Bijvoet JM (1951) The Fourier Synthesis of the Crystal Structure of Strychnine Sulphate Pentahydrate. Acta Cryst 4: 275–280
Bragg L, Perutz MF (1954) The Structure of Haemoglobin VI. Fourier Projection on the 010 Plane. Proc Roy Soc London 225: 315–329
Kendrew JC, Dickerson RE, Strandberg BE et al. (1960) Structure of Myoglobin. A Three-dimensional Fourier Synthesis at 2 Å Resolution. Nature 185: 422–427
Blake CCF, Koenig DF et al., Phillips DC (1965) Structure of Hen Egg-white Lysozyme. A Three-dimensional Fourier Synthesis at 2Å Resolution. Nature 206: 757–761
Michel H (1982) Three-dimensional Crystals of a Membrane Protein Complex. J Mol Biol 158: 567–572
Derrington IM, Butler TZ, Collins MD et al. (2010) Nanopore DNA sequencing with MspA. Proc Natl Acad Sci USA 107: 16060–16065
Faller M, Niederweis M, Schulz GE (2004) The structure of a mycobacterial outer membrane channel. Science 303: 1189–1192
Astbury WT, Bell FO (1938) X-Ray Study of Thymonucleic Acid. Nature 141: 747–748
Chargaff E, Lipshitz R, Green C et al. (1951) The Composition of the Desoxyribonucleic Acid of Salmon Sperm. J Biol Chem 192: 223–230
Cramer F, von der Haar F, et al. Schulz GE (1970) Crystallization of phenylalanine specific transfer ribonucleic acid. J Mol Biol 51: 523–530
Kim SH, Quigley GJ et al. Rich A (1973) Three-Dimensional Structure of Yeast Phenylalanine Transfer-RNA. Folding of the Polynucleotide Chain. Science 179: 285–288
Schulz GE (1974) Wird die Röntgenstrukturanalyse von Proteinen überflüssig? Nachr Chem Techn 22: 431–432
Schulz GE, Barry CD, Friedmann J et al. (1974) Comparison of predicted and experimentally determined secondary structure of adenylate kinase. Nature 250: 140–142
Moult J, Pedersen JT, Judson R et al. (1995) A Large-Scale Experiment to Assess Protein Structure Prediction Methods. Proteins: Struc Func Genet 23: ii–v
Jumper J, Evans R et al. Hassabis D (2021) Highly Accurate Protein Structure Prediction with AlphaFold. Nature 596: 583–589
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Georg E. Schulz 1958–1964 Physikstudium an TU Berlin und der Universität Heidelberg. 1966 Promotion in Physik bei Prof. Dr. O. Haxel an der Universität Heidelberg. 1967–1968 Postdoc bei Dr. H. Wyckoff an der Yale University, USA. 1968–1983 Mitarbeiter in der Abteilung Holmes im Max-Planck-Institut für Medizinische Forschung in Heidelberg. 1983 Habilitation in Biophysik an der Universität Heidelberg. 1984–2007 C4-Professor für Biochemie an der Universität Freiburg. Seit 1998 Mitglied der Leopoldina.
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Schulz, G.E. Die Wege zu den Raumstrukturen von Proteinen und Nukleinsäuren. Biospektrum 29, 118–122 (2023). https://doi.org/10.1007/s12268-023-1909-8
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DOI: https://doi.org/10.1007/s12268-023-1909-8