Abstract
Distant metastasis is the major cause of cancer-related death in virtually all tumour types. We used spontaneous metastasis xenograft mouse models to study differences between primary tumours and corresponding metastases at the proteome level. Sampling of tissues for proteomics with mechanical homogenization is critical, because by this process many proteins are getting lost. For improving the protein yield infrared laser ablation was applied for sampling.
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Hartmut Schlüter 1981–1991 Chemiestudium und Promotion in Biochemie an der Universität Münster, dort 1991–1996 Habilitation im Fach Pathobiochemie. 1996–2000 Ruhr-Universität Bochum. 2000–2008 Charité, Berlin. 2003 Apl. Professur. Seit 2008 Professur für Massenspektrometrische Proteomanalytik am Universitätsklinikum Hamburg-Eppendorf. Seit 2017 Mitglied im Institut für Biochemie, Universität Hamburg.
Tobias Lange 2003–2009 Humanmedizinstudium und Promotion am Universitätsklinikum Hamburg-Eppendorf (UKE), dort 2010–2013 wissenschaftlicher Mitarbeiter am Institut für Anatomie und Experimentelle Morphologie und 2011–2014 MD/PhD-Studium und Promotion. Seit 2013 Juniorprofessor für Translationale Krebsforschung am UKE, 2015 Facharzt für Anatomie. Seit 2017 stellvertretender Institutsdirektor des Instituts für Anatomie und Experimentelle Morphologie, UKE.
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Lange, T., Schlüter, H. Probennahme per Laserablation von Krebsgeweben für Proteomics. Biospektrum 25, 32–34 (2019). https://doi.org/10.1007/s12268-019-1000-7
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DOI: https://doi.org/10.1007/s12268-019-1000-7