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Hyperosmotic Adaptation of Pseudomonas protegens SN15-2 Helps Cells to Survive at Lethal Temperatures

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  • Applied Microbiology
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Abstract

Pseudomonas protegens is a gram-negative bacterium with an excellent biological control effect. Compared to standard cells growing in NaCl-free media, the ability of the hyperosmotic cells (450 mM NaCl) to resist high temperatures and freezing was significantly improved. It is of great significance to apply the P. protegens to elaborate on the hyperosmotic adaptation mechanism. RNA-seq was used to sequence P. protegens cultured with 0 mM and 450 mM NaCl. Comparative transcriptomic analyses of the different treatments were performed using gene ontology and the Kyoto encyclopedia of genes and genome. The results revealed that hyperosmotic stress had prominent impacts on the genes involving in multiple cellular functions. The hyperosmotic environment significantly affected carbohydrate, energy, and amino acid metabolism, as well as membrane system and cell motility. Our findings indicated that P. protegens adopted a series of approaches, including the high cytoplasmic concentrations of potassium ions and the uptake or synthesis of osmoprotectants, for surviving hyperosmotic stress. Among these, trehalose and proline synthesis appeared to be an important method to withstand prolonged hyperosmotic stress in P. protegens. These data provide crucial resource that may determine specific responses to the hyperosmotic environment in P. protegens.

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Acknowledgements

Funding: This work was supported by the National Key Research and Development Program of China under Grant number 2017YFD020040; the Project of Prospering Agriculture through Science and Technology of Shanghai, China under Grant number Hu NongKeChuangZi (2018) No. 2–5.

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  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Xiaobing Wang, Danyan Tang & Wei Wang

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Correspondence to Wei Wang.

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Wang, X., Tang, D. & Wang, W. Hyperosmotic Adaptation of Pseudomonas protegens SN15-2 Helps Cells to Survive at Lethal Temperatures. Biotechnol Bioproc E 25, 403–413 (2020). https://doi.org/10.1007/s12257-019-0430-x

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