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Semi-solid state fermentation of food waste for production of Bacillus thuringiensis biopesticide

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Abstract

In this study, Bacillus thuringiensis (Bt) biopesticide was produced through different fermentation processes using food waste with different water contents. The semi-solid state sample with 75% water content presented the highest δ-endotoxin efficiency of 862 μg/mL. δ-endotoxin efficiency increased by 30.2% from that of the solid-state sample (50% water content) and 73.8% from that of the submerged sample (99% water content). Results confirmed that semi-solid state fermentation presents considerable advantages compared with other fermentation types (solid-state and submerged). The timing adjustment of pH and recycling fermentation effectively counteracted the inhibition of pH and product, thereby increasing δ- endotoxin efficiency to 1,648 and 2,478 μg/mL (accumulated value of all four fermentation loops), respectively. A δ- endotoxin slow-release formulation using polylactic acid as the carrier was also developed to effectively promote the stability of Bt biopesticide.

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Authors and Affiliations

  1. Solid Waste Management Research Institute, Beijing Municipal Research Institute of Environmental Protection, Beijing, 100-037, China

    Wenyu Zhang, Hui Zou, Lin Jiang, Juejun Yao & Jing Liang

  2. Department of Environmental Engineering, University of Science and Technology Beijing, Beijing, 100-083, China

    Qunhui Wang

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  1. Wenyu Zhang
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  2. Hui Zou
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  3. Lin Jiang
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Correspondence to Juejun Yao.

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Zhang, W., Zou, H., Jiang, L. et al. Semi-solid state fermentation of food waste for production of Bacillus thuringiensis biopesticide. Biotechnol Bioproc E 20, 1123–1132 (2015). https://doi.org/10.1007/s12257-015-0347-y

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  • DOI: https://doi.org/10.1007/s12257-015-0347-y

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