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rational design and directed evolution of CYP102A1 (BM3) for regio-specific hydroxylation of isoflavone

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Abstract

Isoflavonoids are a class of polycyclic aromatic hydrocarbon compounds originating from plants as secondary metabolites. Their oxidative metabolites such as ortho dihydroxyisoflavones (ODIs), where the ortho carbons of the hydroxyl groups are hydroxylated, have been reported to have a remarkably high antioxidant effect. Here, we report enzyme engineering of the cytochrome P450 enzyme CYP102A1 (BM3) for the synthesis of ODIs from isoflavonoids.

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Authors and Affiliations

  1. School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Korea

    Sungghi Ko & Byung-Gee Kim

  2. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Korea

    Yung-Hun Yang

  3. Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, 443-749, Korea

    Kwon-Young Choi

  4. Graduate school of Environment and Safety Engineering, Ajou University, Suwon, 443-749, Korea

    Kwon-Young Choi

Authors
  1. Sungghi Ko
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  2. Yung-Hun Yang
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  3. Kwon-Young Choi
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  4. Byung-Gee Kim
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Correspondence to Kwon-Young Choi or Byung-Gee Kim.

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Ko, S., Yang, YH., Choi, KY. et al. rational design and directed evolution of CYP102A1 (BM3) for regio-specific hydroxylation of isoflavone. Biotechnol Bioproc E 20, 225–233 (2015). https://doi.org/10.1007/s12257-014-0718-9

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  • DOI: https://doi.org/10.1007/s12257-014-0718-9

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