Abstract
Streptomyces sp. strain BCNU 1001 was isolated from forest soil samples. Cultural, morphological, and physiological characteristics as well as 16S rDNA analysis revealed that the isolate, BCNU 1001, belonged to the genus Streptomyces. The antimicrobial activity of the ethyl acetate extract was confirmed using the broth microdilution technique. The minimum inhibitory concentration (MIC) of the BCNU 1001 ethyl acetate extract was 0.25 mg/mL for Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa, and 0.125 mg/mL for Micrococcus luteus, Staphylococcus aureus, and Pseudomonas fluorescens. The MIC of the BCNU 1001 ethyl acetate extract for Aspergillus niger, Candida albicans, and Saccharomyces cerevisiae was 0.5, 0.125, and 0.25 mg/mL, respectively. BCNU 1001 was also active against dermatophytic fungi such as Trichophyton mentagrophytes and T. rubrum. Furthermore, BCNU 1001 was also found to be effective against Methicillin-resistant Staphylococcus aureus (MRSA), and its ethyl acetate extract showed MIC = 0.5 mg/mL against MRSA. The most abundant antimicrobial compound was identified as a 2-hydroxybenzyl alcohol through analysis utilizing a nuclear magnetic resonance spectroscopy. This compound was seen to be very effective against some kinds of bacteria and fungi.
Similar content being viewed by others
References
Baker, D. D. and K. A. Alvi (2004) Small-molecule natural products: New structures, new activities. Curr. Opin. Biotechnol. 15: 576–583.
Bull, A. T. and J. E. M. Stach (2007) Marine actinobacteria: New opportunities for natural product search and discovery. Trends Microbiol. 15: 491–499.
Philippon, A., J. Dusart, B. Joris, and J. M. Frere (1998) The diversity, structure and regulation of ß-lactamases. Cell Mol. Life Sci. 54: 341–346.
Pop-Vicas, A. E. and E. M. D’Agata (2005) The rising influx of multidrug-resistant gram negative bacilli into a tertiary care hospital. Clin. Infect. Dis. 40: 1792–1798.
Falagas, M. E. and I. A. Bliziotis (2007) Pandrug-resistant Gramnegative bacteria: The dawn of the post-antibiotic era? Int. J. Antimicrob. Agents 29: 630–636.
Falagas, M. E., P. I. Rafailidis, D. K. Matthaiou, S. Virtzili, D. Nikita, and A. Michalopoulos (2008) Pandrug-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii infections: Characteristics. Int. J. Antimicrob. Agents 32: 450–454.
Shirling, J. L. and D. Gottlieb (1966) Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313–340.
Williams, S. T. and T. Cross (1971) Isolation, purification, cultivation and preservation of actinomycetes. Methods Microbiol. 4: 295–334.
Pridham, T. G. and D. Gottlieb (1948) The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J. Bacteriol. 56: 107–114.
Holding, A. J. and J. G. Collee (1971) Routine biochemical tests. Methods Microbiol. 6: 1–31.
Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3: 208–218.
Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.
Saitou, N. and M. Nei (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.
Charteris, W. P., P. M. Kelly, L. Morelli, and J. K. Collins (1998) Antibiotic susceptibility of potentially probiotic Lactobacillus species. J. Food. Prot. 61: 1636–1643.
Korsten. L., E. S. De Jager, E. E. De Villiers, A. Lourens, and F. C. Wehner (1995) Evaluation of bacterial epiphytes isolated from avocado leaf and fruit surfaces for biocontrol of avocado postharvest diseases. Plant Dis. 79: 1149–1156.
Olano, C., C. Méndez, and J. A. Salas (2009) Antitumor Compounds from Marine Actinomycetes. Mar. Drugs 7: 210–248.
Kohsaka, M., H. Aoki, and H. Imanaka (1987) FK-506, a novel immunosuppressant isolated from a Streptomyces. I. Fermentation, isolation, and physico-chemical and biological characteristics. J. Antibiot. 40: 1249–1255.
Dhiman, S. B., J. P. Kamat, and D. B. Naik (2009) Antioxidant activity and free radical scavenging reactions of hydroxybenzyl alcohols. Biochemical and pulse radiolysis studies Chem. Biol. Interact. 182: 119–127.
Alfaro, C., A. Urios, M. C. González, P. Moya, and M. Blanco (2003) Screening for metabolites from Penicillium novae-zeelandiae displaying radical-scavenging activity and oxidative mutagenicity: Isolation of gentisyl alcohol. Mutat. Res. 539: 187–194.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, H.J., Kim, D.W., Choi, Y.W. et al. Broad-spectrum In vitro antimicrobial activities of Streptomyces sp. strain BCNU 1001. Biotechnol Bioproc E 17, 576–583 (2012). https://doi.org/10.1007/s12257-011-0151-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12257-011-0151-2