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A-Kinase Anchoring Proteins 10 Expression in Relation to 2073A/G Polymorphism and Tumor Progression in Patients with Colorectal Cancer

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Pathology & Oncology Research

Abstract

The cAMP/PKA signalling events regulated by A-kinase anchoring proteins 10 (AKAP10) is involved in tumorigenesis. Previous study showed that AKAP10 polymorphism (2073 A/G, I646V) was associated with colorectal cancer risk. However, there was no literature reporting the role of AKAP10 in the pathogenesis of colorectal cancer. The aim of the study was to investigate the clinicopathologic significance of A-kinase anchoring proteins 10 (AKAP 10) expression and the relationship with its polymorphism in colorectal cancer. The expression of AKAP10 was determined by immunohistochemical staining (IHC) and western blot assay on colorectal cancer (n = 176), adenoma (n = 87) and distant normal mucosa (n = 72). 176 patients with colorectal cancer were genotyped for AKAP10 2073A/G polymorphism by TaqMan RT-PCR. We found that the positive expression rate of AKAP10 in colorectal cancer (59 %) was significantly higher than those in adenoma (39 %) and distant normal mucosa (42 %) (P = 0.004). There was no significant difference between adenoma and distant normal mucosa (P = 0.741). Positive AKAP10 staining was correlated with deeper tumor invasion (P < 0.001), lymph nodes metastasis (P = 0.022), advanced tumor stage (P < 0.001) and poorly differentiated degree (P = 0.003). Compared with AA genotype (52 %), positive expression of AKAP10 was significantly increased in colorectal cancer patients with the variant (AG+GG) genotypes (68 %, P = 0.033). It was concluded that AKAP10 may play an important role in the development and progression of colorectal cancer.

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Acknowledgments

We thank Professors Zhou and Sun for guiding this study, the members of Digestive Surgery Institution of West China Hospital for useful assistance and colleagues of the Department of Gastrointestinal Surgery for providing experimental specimens. This study was supported by the National Natural Science Founding of China (No. 30830100).

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, Institute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, China

    Mojin Wang, Dan Zhang, Yuanyi Rui, Jin Zhou, Rong Wang, Bin Zhou, Lie Yang, Jiankun Hu & Zongguang Zhou

  2. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, China

    Rui Wang

  3. Department of Pathology, West China Hospital, Sichuan University, Chengdu, China

    Xiaoran Huang

  4. Department of Pediatric Surgery, Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China

    Yuan Li

  5. Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, University of Linköping, Linköping, Sweden

    Xiaofeng Sun

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  1. Mojin Wang
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  2. Dan Zhang
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  3. Rui Wang
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  5. Jin Zhou
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  7. Bin Zhou
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  10. Yuan Li
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  11. Jiankun Hu
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  12. Zongguang Zhou
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Correspondence to Zongguang Zhou.

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Wang, M., Zhang, D., Wang, R. et al. A-Kinase Anchoring Proteins 10 Expression in Relation to 2073A/G Polymorphism and Tumor Progression in Patients with Colorectal Cancer. Pathol. Oncol. Res. 19, 521–527 (2013). https://doi.org/10.1007/s12253-013-9612-6

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  • DOI: https://doi.org/10.1007/s12253-013-9612-6

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