Abstract
The duck circovirus (DuCV) infection in sick ducks from Fujian Province was investigated. The liver samples of 43 sick Muscovy ducks with infectious serositis were collected from 12 duck farms in Fujian Province. Based on the published sequences of DuCV, two primers were designed for the detection of DuCV and four pairs of primers were designed to amplify four overlapping fragments that cover the complete genome of DuCV. The specific PCR products were amplified from positive samples. The fragments were then cloned into pMD18-T vector and sequenced, and the full length genomic sequence of the FJ0601 isolate of DuCV was obtained. PCR analysis showed that the proportion of ducks which were positive for circovirus was 79% and 10 out of the 12 farms were positive. Sequence analysis showed that the complete genome of DuCV-FJ0601 was 1988 bp and possessed features common to the family Circoviridae which included a stem-loop structure and the Rep protein motifs. Homology analysis showed that FJ0601 isolate of DuCV had 97.3%∼97.5% nucleotide sequence identity to all the four Taiwan isolates (TC1/2002, TC2/2002, TC3/2002, TC4/2002), 82.9% identity to the America (33753-52) isolate and 82.3% identity to the Germany isolate. Phylogenetic analysis with Clustal W, however, showed that FJ0601 isolate of DuCV was on a common branch with Taiwan isolates, and Germany and America isolates belonged to the other branch.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allan G M, Ellis J A. 2000. Procine circoviruses: a review. J Vet Diagn Invest, 12(1): 3–14.
Banda A, Galloway-Haskins R I, Sandhu T S, et al. 2007. Genetic analysis of a duck circovirus detected in commercial Pekin ducks in New York. Avian Dis, 51(1):90–95.
Bassami M R, Berryman D, Wilcox G E, et al. 1998. Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. Virology, 249(2): 453–459.
Chen C L, Wang P X, Lee M S, et al. 2006. Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. Avian Dis, 50(1): 92–95.
ClarkE G. 1997. Postweaning multisystemic wasting syndrome. Proceeding of the Americans Association of swine practitioners, 499–501.
Eisenberg S W, Asten van H J, Ederen van A M, et al. 2003. Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands. Vet Microbiol, 95: 27–38.
Fringuelli E, Scott A N, Beckett A, et al. 2005. Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests. Avian Pathol, 34(6): 495–500.
Hattermann K, Schmitt C, Soike D, et al. 2003. Cloning and sequencing of Duck circovirus (DuCV). Arch Virol, 148(12): 2471–2480.
Mankertz A, Hattermann K, Ehlers B, et al. 2000. Cloning and sequencing of columid circovirus(CoCV), a new circovirus from pigeons. Arch Virol, 145(12): 2469–2479.
Phenix K V, Weston J H, Ypelaar I, et al. 2001. Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. J Gen Virol, 82(11):2805–2809.
Pringle C R. 1999. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia. Arch Virol, 144(10): 2065–2070.
Raidal S R, Riddoch P A. 1997. A feather disease in Senegal doves (Streptopelia senegalensis) morphologically similar to psittacine beak and feather disease. Avian Pathol, 26: 829–836.
Shivaprasad H L, Hill L D, Todd D, et al. 2004. Circovirus infection in a Gouldian finch (Chloebia gouldiae). Avian Pathol, 33: 525–529.
Soike D, Albrecht K, Hattermann K, et al. 2004. Novel circovirus in mulard ducks with developmental and feathering disorders. Vet Rec, 154: 792–793.
Tischer I, Gelderblom H, Vettermann W, et al. 1982. A very small porcine circovirus with circular single-stranded DNA. Nature, 295(7): 64–66.
Todd D. 2000. Circoviruses: immunosuppressive threats to avian species: a review. Avian Pathol, 29(6): 373–394.
Todd D, Weston J H, Soike D, et al. 2000. Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology, 286(2): 354–362.
Todd D. 2004. Avian circovirus diseases: lessons for the study of PMWS. Vet Microbiol, 98(2): 169–174.
Twentyman C M, Alley M R, Meers J, et al. 1999. Circovirus-like infectionin a southern black-bached dull (Larus dominicanus). Avian Pathol, 28: 513–516.
Yuasa N, Taniguchi T, Yoshida I. 1979. Isolation and some characteristics of an agent inducing anemia in chicks. Avian Dis, 23: 366–385.
Yuasa N. 1983. Propagation and infectivity titration of the Gifu-1strain of chicken anemia agent in a cell line (MDCC-MSB1) derived from Marek’s disease lymphoma. Natl Inst Animal Health Q, 23: 13–20.
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation items: Shandong Provincial Excellent Young Scientist Fund (2004BS06002); Innovation Fund of Shandong Agricultural University
Rights and permissions
About this article
Cite this article
Jiang, Sj., Zhang, Xx., Liu, Sn. et al. PCR detection and sequence analysis of duck circovirus in sick Muscovy ducks. Virol. Sin. 23, 265–271 (2008). https://doi.org/10.1007/s12250-008-2934-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12250-008-2934-y