Skip to main content
SpringerLink
Log in

Karyological characterisation of four American species of Crotalaria (Leguminosae: Papilionoideae) by the splash method

  • Published:
Kew Bulletin Aims and scope Submit manuscript

Summary

The karyotypes of Crotalaria juncea L., C. sagittalis L. (sect. Calycinae Wight & Arn.), C. mollicula Kunth and C. pumila Ortega (sect. Crotalariae Baker) were obtained by a surface-spreading and air-drying method (splash). All species have chromosome numbers based on x = 8 (2n = 16, 32). Chromosomes are metacentric (m) or submetacentric (sm), with one pair of satellite-chromosomes per complement. Although similar, the karyotypes are not particularly uniform, supporting the view that inter-specific differences of karyotypes can be used in species characterisation. There is sufficient evidence to show that large structural chromosome changes and hybridisation are significant in the diversification of this genus, particularly in the New World.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Almada, R. D., Daviña, J. R. & Seijo J. G. (2006). Karyotype analysis and chromosome evolution in southernmost South American species of Crotalaria (Leguminosae). Bot. J. Linn. Soc. 150: 329 – 341.

    Article  Google Scholar 

  • Arellano, J. (1986). Etnobotánica de leguminosas: notas sobre algunas de las especies utilizadas en la alimentación en México. Anales Inst. Biol. Univ. Nac. Autón. Mexico, Bot. 57: 123 – 142

    Google Scholar 

  • Atchison, E. (1950). Studies in the Leguminosae. V. Cytological observations on Crotalaria. J. Elisha Mitchell Sci. Soc. 66: 70 – 75.

    Google Scholar 

  • Boulter, D., Derbyshire, E., Frahm-Leliveld, J. A. & Polhill, R. M. (1970). Observations on the cytology and seed proteins of various African species of Crotalaria L. (Leguminosae). New Phytol. 69: 117 – 131.

    Article  Google Scholar 

  • Chennaveeraiah, M. S. & Patil, B. C. (1973). Chromosome number and karyotype study in eight species of Crotalaria. Cytologia 38: 73 – 79.

    Article  Google Scholar 

  • Cotias de Oliveira, A. L. P. & Aguiar-Perecin, M. L. R. (1999). Karyotype evolution in the genus Crotalaria (Leguminosae). Cytologia 64: 165 – 174.

    Article  Google Scholar 

  • D’Ovidio, R. & Marchi, P. (1990). DNA content, karyotype structure analysis and karyotype symmetry in Ranunculus L. (Ranunculaceae). Italian species belonging to sections Flammula (Webb) Benson and Micranthus (Ovcz) Nyarady. Caryologia 43: 99 – 115.

    Google Scholar 

  • Datta, R. M. & Choudhury, P. C. (1966). Karyotype in Crotalaria. Bull. Torrey Bot. Club 93: 241 – 243.

    Article  Google Scholar 

  • Fernández, A. (1977). Números cromosómicos en Angiospermas. Hickenia 15: 83 – 86.

    Google Scholar 

  • Flores, A. S., Corrêa, A. M., Forni-Martins, E. R. & Tozzi, A. M. G. A. (2006). Chromosome numbers in Brazilian species of Crotalaria (Leguminosae, Papilionoideae) and their taxonomic significance. Bot. J. Linn. Soc. 151: 271 – 277.

    Article  Google Scholar 

  • Gómez-Sosa, E. (2000). Las especies argentinas de Crotalaria (Leguminosae-Crotalarieae): novedades, descripciones y claves. Gayana Botánica 57: 67 – 87.

    Article  Google Scholar 

  • Gupta, R. & Gupta, P. K. (1978). Karyotypic studies in the genus Crotalaria Linn. Cytologia 43: 357 – 369.

    Article  Google Scholar 

  • Kundu, A. K. & Sharma, A. K. (1985). Chromosome characteristics and DNA content in Mentha Linn. The Nucleus 28: 89 – 96.

    Google Scholar 

  • Le Roux, M. M., Van Wick, B-E., Boatwright, J. S. & Tilney, P. M. (2011). The systematic significance of morphological and anatomical variation in fruits of Crotalaria and related genera of tribe Crotalarieae. Bot. J. Linn. Soc. 165: 84 – 106.

    Article  Google Scholar 

  • Levan, A., Fredga, K. & Sandberg, A. A. (1964). Nomenclature for centromeric position on chromosome. Hereditas 52: 201 – 219.

    Article  Google Scholar 

  • Magoon, M. L., Koppar, M. N., Ramanna, M. S. & Shina, A. K. (1963). Cytomorphological studies in genus Crotalaria. La Cellule 63: 375 – 396.

    Google Scholar 

  • Mangotra, R. & Koul, A. K. (1991). Polyploidy in genus Crotalaria. Cytologia 56: 293 – 296.

    Article  Google Scholar 

  • Navashin, M. S. (1928). Amphiplastie – eine neue karyologische Erscheinung. Proc. Int. Conf. Genet. 5: 1148 – 1152.

    Google Scholar 

  • Palomino, G. & Vázquez, R. (1991). Cytogenetic studies in Mexican populations of species of Crotalaria L. (Leguminosae-Papilionoideae). Cytologia 56: 343 – 351.

    Article  Google Scholar 

  • Polhill, R. M. (1968). Miscellaneous notes on African species of Crotalaria L. II. Kew Bull. 22: 169 – 337.

    Article  Google Scholar 

  • ____ (1981). Crotalarieae. pp. 399 – 401. In: R. M. Polhill & P. H. Raven (eds.) Advances in Legume Systematics I. Royal Botanic Gardens, Kew.

    Google Scholar 

  • Preuss, S. & Pikaard, C. S. (2007). rRNA gene silencing and nucleolar dominance: Insights into a chromosme-scale epigenetic on/off switch. Biochim. Biophys. Acta 1769: 383 – 392.

    Article  PubMed  CAS  Google Scholar 

  • Raina, S. N. & Verma, R. C. (1979). Cytogenetics of Crotalaria I. Mitotic complements in twenty species of Crotalaria L. Cytologia 44: 365 – 375.

    Article  Google Scholar 

  • Samuel, R., Smith, J. B. & Bennett, M. D. (1986). Nuclear DNA variation in Piper (Piperaceae). Canad. J. Genet. Cytol. 28: 1041 – 1043.

    Google Scholar 

  • Sharma, A. K. (1970). Polyploidy and chromosome size. Chromosomes Today 3: 248 – 252.

    Google Scholar 

  • Sharma, A. & Sen, S. (2002). Chromosome Botany, pp. 92 – 99. Science Publishers, Inc. Enfield, NH.

    Google Scholar 

  • ____ & Sharma, A. (1984). Trends in chromosome evolution in the plant kingdom. In: A. K. Sharma (ed.), Chromosomes in evolution of eukaryotic groups 2, pp. 227 – 239. CRC Press, New Delhi.

    Google Scholar 

  • Sousa, S. M. & Delgado, A. (1998). Leguminosas mexicanas: fitogeografía, endemismo y orígenes. In: T. P. Ramamoorthy, R. Bye, A. Lot & J. Fa (eds), Diversidad biológica de México: orígenes y distribución, pp. 449 – 500. Instituto de Biología, Universidad Nacional Autónoma de México.

  • Tapia-Pastrana, F. & Mercado-Ruaro, P. (2001). A combination of the ‘squash’ and ‘splash’ techniques to obtain the karyotype and assess meiotic behavior in Prosopis laevigata L. (Fabaceae: Mimosoideae). Cytologia 66: 11 – 17.

    Article  Google Scholar 

  • ____, Gallegos-Pacheco, E., de Teodoro-Pardo, C. & Mercado-Ruaro, P. (2005). New cytogenetic information of two Mexican populations of Crotalaria incana L. (Leguminosae-Papilionoideae). Cytologia 70: 207 – 212.

    Article  Google Scholar 

  • Turner, B. & Fearing, O. (1960). Chromosome numbers in the Leguminosae III. Species of the southwestern United States and Mexico. Amer. J. Bot. 47: 603 – 608.

    Article  Google Scholar 

  • Verma, R. C. & Raina, S. N. (1983). Cytogenetics of Crotalaria VIII. Male meiosis in 26 species. Cytologia 48: 719 – 733.

    Article  Google Scholar 

  • ____, Kesavacharyulu K. & Raina, S. N. (1984). Cytogenetics of Crotalaria IX. Mitotic complements in nineteen species. Cytologia 49: 157 – 169.

    Article  Google Scholar 

  • Ward, D. E. (1983). Chromosome counts from New Mexico and southern Colorado. Phytologia 54: 302 – 309.

    Google Scholar 

  • Windler, D. R. (1974). Chromosome numbers for native North American unifoliolate species of Crotalaria (Leguminosae). Brittonia 26: 172 – 176.

    Article  Google Scholar 

Download references

Acknowledgements

The author thanks Alfonso Delgado-Salinas, Instituto de Biología UNAM, for critical revision and valuable comments.

Author information

Authors and Affiliations

  1. Laboratorio de Genecología, División de Estudios de Posgrado e Investigación Facultad de Estudios Superiores Zaragoza, UNAM, Apartado Postal 9-020, 15000, México, D. F, México

    Fernando Tapia-Pastrana

Authors
  1. Fernando Tapia-Pastrana
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fernando Tapia-Pastrana.

Appendix

Appendix

Details of material examined:

Crotalaria juncea. México, Cuixmala, Jalisco 19°28'N, 104°58'W; 110 m alt.. Gómez & Trejo 71 (MEXU).

Crotalaria mollicula. El Salvador, Mpio. Texistepeque. Santa Ana. 14°11'06"N. 89°28'02"W; 450 m alt. Linares & Martínez s.n (MEXU).

Crotalaria sagittalis. México, Morelos 18°56'N, 99°13'W; 1680 m. Tapia & Gómez 58 (MEXU).

Crotalaria pumila. México, D.F., Reserva Ecológica del Pedregal de San Ángel, 19°16'N; 99°10'W; 2263 m alt. Tapia & Gómez 59 (MEXU).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tapia-Pastrana, F. Karyological characterisation of four American species of Crotalaria (Leguminosae: Papilionoideae) by the splash method. Kew Bull 67, 427–433 (2012). https://doi.org/10.1007/s12225-012-9385-1

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12225-012-9385-1

Key Words

Search

Navigation