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Association of interleukin-10 gene promoter polymorphisms with susceptibility to acute pyelonephritis in children

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Abstract

Interleukin-10 (IL-10) is a potent inhibitor of leukocyte chemotaxis, bacterial killing in phagocytes and synthesis of pro-inflammatory cytokines and chemokines, and recent studies have suggested an important role for this immunoregulatory cytokine in the pathogenesis of urinary tract infections (UTIs). Therefore, the gene encoding IL-10 (IL10) is an attractive candidate for association studies attempting to identify susceptibility genes conferring risk of UTIs. In this case–control study, we aimed to investigate the association of single nucleotide polymorphisms (SNPs) in the promoter region of IL10 with acute pyelonephritis in the Slovak population. Polymerase chain reaction with sequence-specific primers was used to analyse IL10 −1082A/G (rs1800896), −819C/T (rs1800871) and −592C/A (rs1800872) SNPs in 147 children with acute pyelonephritis and 215 healthy controls. Comparison of patients with healthy controls using the logistic regression analysis revealed significantly increased risk of developing recurrent attacks of acute pyelonephritis for −1082 G allele in a dominant genetic model GG (GG + AG vs. AA, P = 0.019, odds ratio (OR) = 2.26). A similar tendency was also found when the recurrent acute pyelonephritis subgroup was compared to episodic pyelonephritis cases (GG + AG vs. AA, P = 0.009, OR = 3.38). In conclusion, our results suggest that IL10 −1082 A/G SNP is a susceptibility factor for development of recurrent attacks of acute pyelonephritis.

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Acknowledgments

This work was financially supported by Slovak Research and Development Agency grant APVV-0117-07. The authors are grateful to Mrs. B. Faragová and Ing. J. Kostolanská, Ph.D. for their technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, Comenius University in Bratislava, Odborárske nám. 14, 81108, Bratislava, Slovakia

    Juraj Javor, Mária Bucová, Milan Buc & Adriana Liptáková

  2. 3rd Pediatric Clinic of Slovak Medical University, General Hospital Lučenec, Nám. republiky 15, 98439, Lučenec, Slovakia

    Karol Králinský

  3. Nephrology Clinic NefroSa Ltd., Srbská 1, 04001, Košice, Slovakia

    Eva Sádová

  4. 1st Department of Pediatrics, Faculty of Medicine, Comenius University in Bratislava and University Children’s Hospital, Limbová 1, 83340, Bratislava, Slovakia

    Oľga Červeňová

  5. 5th Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava and University Hospital, Ružinovská 6, 82606, Bratislava, Slovakia

    Michaela Olejárová

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  1. Juraj Javor
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  2. Karol Králinský
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  3. Eva Sádová
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  4. Oľga Červeňová
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  5. Mária Bucová
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  6. Michaela Olejárová
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  7. Milan Buc
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  8. Adriana Liptáková
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Correspondence to Juraj Javor.

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Javor, J., Králinský, K., Sádová, E. et al. Association of interleukin-10 gene promoter polymorphisms with susceptibility to acute pyelonephritis in children. Folia Microbiol 59, 307–313 (2014). https://doi.org/10.1007/s12223-014-0303-9

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  • DOI: https://doi.org/10.1007/s12223-014-0303-9

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