Abstract
A rapid method that included simple boiling DNA extraction followed by a fast polymerase chain reaction (PCR) cycling protocol designed to detect mecA, which characterizes methicillin-resistant Staphylococcus aureus (MRSA), was performed. Briefly, the PCR cycling protocol consisted of pre-denaturation at 95 °C for 30 s, 30 cycles of denaturation at 94 °C for 2 s, annealing at 52 °C for 5 s, extension for 10 s, and final extension at 72 °C for 1 min. A good level of reliability of the method was verified. The study has shown that the method described here represents a rapid and accurate DNA extraction and PCR-based identification system of MRSA, thus allowing clinicians to make early identification and early implementation of control measures.
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Abbreviations
- PCR:
-
Polymerase chain reaction
- mecA :
-
Methicillin-resistant gene
- MRSA:
-
Methicillin-resistant Staphylococcus aureus
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Adwan, K. Fast DNA isolation and PCR protocols for detection of methicillin-resistant staphylococci. Folia Microbiol 59, 5–8 (2014). https://doi.org/10.1007/s12223-013-0259-1
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DOI: https://doi.org/10.1007/s12223-013-0259-1