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Hypoglycemic and hypolipidemic effects of sugar beet (Beta vulgaris L.) extract on C57BL/KsJ db/db mice

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Abstract

Sugar beet (Beta vulgaris L. (BV)) is one of the most important economic crops. The purpose of this study was to examine the effects and mechanism of BV on the glucolipid metabolism. The study was conducted on C57BL/KsJ db/db mice, and the experiments were divided into four groups: diabetic control (distilled water, db/db-Veh), metformin treatment (metformin, db/db-Met, 250 mg/kg), and BV treatment (BV, db/db, 1.8 and 3.6 g/kg of BV). The metformin and BV were intragastrically administrated into mice. The results showed that the treatment with BV resulted in the decreased levels of the fasting blood glucose (FBG), uric acid (UA), and lipid, as well as improving the abnormalities of liver and pancreas. The protein levels of insulin receptor substrate‐1 (IRS‐1)/PI3K/AKT and AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) were quantified by Western blotting. The results showed that the hypoglycemic effect was partly due to the activation of the IRS‐1/PI3K/AKT signaling pathway, and the AMPK/ACC signaling pathway was responsible for the hypolipidemic activity. This study demonstrated that BV possesses hypoglycemic and hypolipidemic effects on diabetic db/db mice, indicating that it can be contributed to the treatment of type 2 diabetes.

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Funding

This work was supported by Jiangsu Provincial Natural Science Foundation of China (BK20190233), Science and Technology Develop Project in Changshu (CS202014).

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Authors and Affiliations

  1. College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China

    Zhipan Wu, Yuhang Shen & Jingkui Tian

  2. Institute of Cancer and Basic Medicine, Chinese Academy of Sciences, Zhejiang Cancer Hospital, Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, 310022, China

    Zhipan Wu, Haote Han, Wei Zhu, Jingkui Tian & Shouxin Li

  3. Shandong Drug and Food Vocational College, Weihai, 264210, China

    Haitao Gong

  4. Changshu Qiushi Technology Corporation Limited, Suzhou, 215000, China

    Wei Zhu & Shouxin Li

  5. Agricultural College, Shihezi University, Shihezi, 832003, Xinjiang, China

    Hua Fan

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  1. Zhipan Wu
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  2. Haitao Gong
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  7. Hua Fan
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  8. Shouxin Li
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Contributions

ZPW: writing original draft, data acquisition, analysis, and interpretation. YHS, HTH, and HTG: data acquisition and interpretation. WZ, JKT, HF, and SXL: supervision, project administration, review and editing, funding, and resources’ acquisition.

Corresponding authors

Correspondence to Hua Fan or Shouxin Li.

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The Experiment was approved by the Animal Ethics Committee of Zhejiang University (Approval Number: ZJU20200168).

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Wu, Z., Gong, H., Shen, Y. et al. Hypoglycemic and hypolipidemic effects of sugar beet (Beta vulgaris L.) extract on C57BL/KsJ db/db mice. Rend. Fis. Acc. Lincei 33, 205–212 (2022). https://doi.org/10.1007/s12210-022-01054-z

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  • DOI: https://doi.org/10.1007/s12210-022-01054-z

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