Abstract
Aeolian sand is a fine, non-cohesive and homogeneous material widely distributed in desert areas, and therefore susceptible to wind erosion, causing serious environmental concerns. This study demonstrates the promise of enzyme-induced carbonate precipitation (EICP) as a means of solidifying aeolian sand to prevent wind erosion. Different cementation solution concentration, urease activity, temperature and number of treatments will have diverse influence on the mechanical property of solidified sand. The test-tube experiments were performed to evaluate the effect of these factors on the enzymatic calcium carbonate precipitation process. The method was then applied to solidify aeolian sand to assess the reinforcement effect by unconfined compressive strength (UCS), calcium carbonate content and mercury intrusion capillary pressure tests. The results demonstrated that the increase of urease activity from 2.95 U/mL to 5.39 U/mL and that of cementation solution from 0.25 M to 0.75 M resulted in an increase in UCS. The increase of number of treatments and that of temperature from 15°C to 45°C can also effectively enhance the mechanical property of aeolian sand. The mercury intrusion capillary pressure test revealed that the improvement of performance of solidified aeolian sand was mainly due to the reduction of porosity caused by the generated of calcium carbonate; and there is an exponential function relationship between the strength and porosity. Furthermore, the increase in urease activity and temperature significantly reduced the porosity and the proportion of larger pores in the solidified sand, thereby appreciably enhancing the strength of aeolian sand with a minor increase in calcium carbonate content.
Similar content being viewed by others
References
AlAhmari M, Bataweel M, AlHumam A, AlMajed A (2020) sand consolidation by enzyme mediated calcium carbonate precipitation. Proceedings of Abu Dhabi international petroleum exhibition & conference, November 9–12, Abu Dhabi, UAE
Almajed A, Tirkolaei HK, Kavazanjian E (2018) Baseline investigation on enzyme-induced calcium carbonate precipitation. Journal of Geotechnical and Geoenvironmental Engineering 144(11):04018081, DOI: https://doi.org/10.1061/(ASCE)GT.1943-5606.0001973
Almajed A, Tirkolaei HK, Kavazanjian E, Hamdan N (2019) Enzyme induced biocementated sand with high strength at low carbonate content. Scientific Reports 9(1):1–7, DOI: https://doi.org/10.1038/s41598-018-38361-1
Al Qabany A, Soga K (2014) Effect of chemical treatment used in MICP on engineering properties of cemented soils. Géotechnique 63(4):331–339, DOI: https://doi.org/10.1680/geot.SIP13.P.022
Amarakoon GGNN, Kawasaki S (2018) Factors affecting sand solidification using MICP with pararhodobacter sp. Materials Transactions 59(1):72–81, DOI: https://doi.org/10.2320/matertrans.M-M2017849
Arab MG, Rohy H, Zeiada W, Almajed A, Omar M (2021) One-phase EICP biotreatment of sand exposed to various environmental conditions. Journal of Materials in Civil Engineering 33(3):04020489, DOI: https://doi.org/10.1061/(ASCE)MT.1943-5533.0003596
Carmona JP, Oliveira PJV, Lemos LJ (2016) Biostabilization of a sandy soil using enzymatic calcium carbonate precipitation. Procedia Engineering 143:1301–1308, DOI: https://doi.org/10.1016/j.proeng.2016.06.144
Carmona JP, Oliveira PJV, Lemos LJ, Pedro AM (2018) Improvement of a sandy soil by enzymatic calcium carbonate precipitation. Proceedings of the Institution of Civil Engineers-Geotechnical Engineering 171(1): 3–15, DOI: https://doi.org/10.1680/jgeen.16.00138
Cheng L, Shahin MA, Mujah D (2017) Influence of key environmental conditions on microbially induced cementation for soil stabilization. Journal of Geotechnical and Geoenvironmental Engineering 143(1): 04016083, DOI: https://doi.org/10.1061/(ASCE)GT.1943-5606.0001586
Cui MJ, Lai HJ, Hoang T, Chu J (2021) One-phase-low-pH enzyme induced carbonate precipitation (EICP) method for soil improvement. Acta Geotechnica 16(2):481–489, DOI: https://doi.org/10.1007/s11440-020-01043-2
Dakhane A, Das S, Hansen H, O’Donnell S, Hanoon F, Rushton A, Neithalath N (2018) Crack healing in cementitious mortars using enzyme-induced carbonate precipitation: Quantification based on fracture response. Journal of Materials in Civil Engineering 30(4): 04018035, DOI: https://doi.org/10.1061/(ASCE)MT.1943-5533.0002218
DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Biomediated soil improvement. Ecological Engineering 36(2):197–210, DOI: https://doi.org/10.1016/j.ecoleng.2008.12.029
Dilrukshi RAN, Kawasaki S (2016) Effective use of plant-derived urease in the field of geoenvironmental/geotechnical engineering. Journal of Civil & Environmental Engineering 6(207):2, DOI: https://doi.org/10.4172/2165-784X.1000207
Dilrukshi RAN, Nakashima K, Kawasaki S (2018) Soil improvement using plant-derived urease-induced calcium carbonate precipitation. Soils and Foundations 58(4):894–910, DOI: https://doi.org/10.1016/j.sandf.2018.04.003
Elipe MG, Lopez-Querol S (2014) Aeolian sands: Characterization, options of improvement and possible employment in construction—The state-of-the-art. Construction and Building Materials 73:728–739, DOI: https://doi.org/10.1016/j.conbuildmat.2014.10.008
GB/T 50123 (2019) Standard for geotechnical testing method. GB/T 50123, Ministry of Housing and Urban-Rural Development of the People’s Republic of China, Beijing, China
Hamdan N, Kavazanjian E (2016) Enzyme-induced carbonate mineral precipitation for fugitive dust control. Géotechnique 66(7):546–555, DOI: https://doi.org/10.1680/jgeot.15.P.168
Hamdan N, Kavazanjian E, O’Donnell S (2013) Carbonate cementation via plant derived urease. Proceedings of the 18th international conference on soil mechanics and geotechnical engineering, September 2–5, Paris, France
Harkes MP, Van Paassen LA, Booster JL, Whiffin VS, van Loosdrecht MC (2010) Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecological Engineering 36(2):112–117, DOI: https://doi.org/10.1016/j.ecoleng.2009.01.004
Javadi N, Khodadadi H, Hamdan N, Kavazanjian E (2018) EICP treatment of soil by using urease enzyme extracted from watermelon seeds. IFCEE, March 5–10, Orlando, FL, USA
Jin S, Zhou J, Zhao X, Sun L (2021) Quantitative relationship between pore size distribution and compressive strength of cementitious materials. Construction and Building Materials 273:121727, DOI: https://doi.org/10.1016/j.conbuildmat.2020.121727
Kavazanjian E, Hamdan N (2015) Enzyme induced carbonate precipitation (EICP) columns for ground improvement. IFCEE, March 17–21, San Antonio, TX, USA
Khodadadi TH, Kavazanjian E, van Paassen L, DeJong J (2017) Biogrout materials: A review. Grouting, July 9–12, Honolulu, HI, USA
Kontoyannis CG, Vagenas NV (2000) Calcium carbonate phase analysis using XRD and FT-Raman spectroscopy. Analyst 125(2):251–255, DOI: https://doi.org/10.1039/A908609I
Lee S, Kim J (2020) An experimental study on enzymatic-induced carbonate precipitation using yellow soybeans for soil stabilization. KSCE Journal of Civil Engineering 24(7):2026–2037, DOI: https://doi.org/10.1007/s12205-020-1659-9
Li D, Tian KL, Zhang HL, Wu YY, Nie KY, Zhang SC (2018a) Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation. Construction and Building Materials 172:251–262, DOI: https://doi.org/10.1016/j.conbuildmat.2018.03.255
Li C, Yao D, Liu S, Zhou T, Bai S, Gao Y, Li L (2018b) Improvement of geomechanical properties of bio-remediated Aeolian sand. Geomicrobiology Journal 35(2):132–140, DOI: https://doi.org/10.1080/01490451.2017.1338798
Lian C, Zhuge Y, Beecham S (2011) The relationship between porosity and strength for porous concrete. Construction and Building Materials 25(11):4294–4298, DOI: https://doi.org/10.1016/j.conbuildmat.2011.05.005
Liu KW, Jiang NJ, Qin JD, Wang YJ, Tang CS, Han XL (2021) An experimental study of mitigating coastal sand dune erosion by microbial-and enzymatic-induced carbonate precipitation. Acta Geotechnica 16(2):467–480, DOI: https://doi.org/10.1007/s11440-020-01046-z
Lopez-Querol S, Arias-Trujillo J, Maria GE, Matias-Sanchez A, Cantero B (2017) Improvement of the bearing capacity of confined and unconfined cement-stabilized aeolian sand. Construction and Building Materials 153:374–384, DOI: https://doi.org/10.1016/j.conbuildmat.2017.07.124
Miao LC, Wu LY, Sun XH, Li X, Zhang JZ (2020) Method for solidifying desert sands with enzyme-catalysed mineralization. Land Degradation & Development 31(11):1317–1324, DOI: https://doi.org/10.1002/ldr.3499
Muhammed AS, Kassim KA, Ahmad K, Zango MU, Chong CS, Makinda J (2021) Influence of multiple treatment cycles on the strength and microstructure of biocemented sandy soil. International Journal of Environmental Science and Technology 18:3427–3440, DOI: https://doi.org/10.1007/s13762-020-03073-5
Mujah D, Shahin MA, Cheng L (2017) State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) for soil stabilization. Geomicrobiology Journal 34(6):524–537, DOI: https://doi.org/10.1080/01490451.2016.1225866
Nebel H, Epple M (2008) Continuous preparation of calcite, aragonite and vaterite, and of magnesium-substituted amorphous calcium carbonate (Mg-ACC). Zeitschrift Für Anorganische Und Allgemeine Chemie 634(8):1439–1443, DOI: https://doi.org/10.1002/zaac.200800134
Neupane D, Yasuhara H, Kinoshita N, Putra H (2015) Distribution of grout material within 1-m sand column in insitu calcite precipitation technique. Soils and Foundations 55(6):1512–1518, DOI: https://doi.org/10.1016/j.sandf.2015.10.015
Neupane D, Yasuhara H, Kinoshita N, Unno T (2013) Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique. Journal of Geotechnical and Geoenvironmental Engineering 139(12):2201–2211, DOI: https://doi.org/10.1061/(ASCE)GT.1943-5606.0000959
Pan ZY, Li G, Hong CY, Kuang HL, Yu Y, Feng FX, Liu DM, Du H (2015) Modified recycled concrete aggregates for asphalt mixture using microbial calcite precipitation. RSC Advances 5(44):34854–34863, DOI: https://doi.org/10.1039/C5RA04203H
Park SS, Choi SG, Nam IH (2014) Effect of plant-induced calcite precipitation on the strength of sand. Journal of Materials in Civil Engineering 26(8):06014017, DOI: https://doi.org/10.1061/(ASCE)MT.1943-5533.0001029
Rong H, Qian CX, Li LZ (2012) Study on microstructure and properties of sandstone cemented by microbe cement. Construction and Building Materials 36:687–694, DOI: https://doi.org/10.1016/j.conbuildmat.2012.06.063
Rowshanbakht K, Khamehchiyan M, Sajedi RH, Nikudel MR (2016) Effect of injected bacterial suspension volume and relative density on carbonate precipitation resulting from microbial treatment. Ecological Engineering 89:49–55, DOI: https://doi.org/10.1016/j.ecoleng.2016.01.010
Song JY, Sim Y, Jang J, Hong WT, Yun TS (2019) Near-surface soil stabilization by enzyme-induced carbonate precipitation for fugitive dust suppression. Acta Geotechnica 15:1967–1980, DOI: https://doi.org/10.1007/s11440-019-00881-z
Sumner JB (1926) The isolation and crystallization of the enzyme urease preliminary paper. Journal of Biological Chemistry 69(2): 435–441, DOI: https://doi.org/10.4159/harvard.9780674366701.c115
Sun XH, Miao LC, Wang HX, Yin WH, Wu LY (2021) Mineralization crust field experiment for desert sand solidification based on enzymatic calcification. Journal of Environmental Management 287:112315, DOI: https://doi.org/10.1016/j.jenvman.2021.112315
Sun XH, Miao LC, Wu LY (2020) Applicability and theoretical calculation of enzymatic calcium carbonate precipitation for sand improvement. Geomicrobiology Journal 37(4):389–399, DOI: https://doi.org/10.1080/01490451.2019.1710625
Sun XH, Miao LC, Wu LY, Wang CC (2019) Study of magnesium precipitation based on biocementation. Marine Georesources & Geotechnology 37(10):1257–1266, DOI: https://doi.org/10.1080/1064119X.2018.1549626
Tian K, Wang X, Zhang S, Zhang H, Zhang F, Yang A (2020) Effect of reactant injection rate on solidifying aeolian sand via microbially induced calcite precipitation. Journal of Materials in Civil Engineering 32(10):04020291, DOI: https://doi.org/10.1061/(ASCE)MT.1943-5533.0003391
Van Paassen LA, Ghose R, van der Linden TJ, van der Star WR, van Loosdrecht MC (2010) Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment. Journal of Geotechnical and Geoenvironmental Engineering 136(12):1721–1728, DOI: https://doi.org/10.1061/(ASCE)GT.1943-5606.0000382
Yang J, Wang F, Fang L, Tan T (2007) Synthesis, characterization and application of a novel chemical sand-fixing agent-poly (aspartic acid) and its composites. Environmental Pollution 149(1):125–130, DOI: https://doi.org/10.1016/j.envpol.2006.12.021
Yasuhara H, Hayashi K, Okamura M (2011) Evolution in mechanical and hydraulic properties of calcite-cemented sand mediated by biocatalyst Geo-frontiers 2011: Advances in geotechnical engineering, March 13–16, Dallas, TX, USA
Yasuhara H, Neupane D, Hayashi K, Okamura M (2012) Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation. Soils and Foundations 52(3):539–549, DOI: https://doi.org/10.1016/j.sandf.2012.05.011
Yuan H, Ren G, Liu K, Zheng W, Zhao Z (2020) Experimental study of eicp combined with organic materials for silt improvement in the Yellow River flood area. Applied Sciences 10(21):7678, DOI: https://doi.org/10.3390/app10217678
Zhang ZJ, Tong KW, Hu L, Yu Q, Wu LL (2020a) Experimental study on solidification of tailings by MICP under the regulation of organic matrix. Construction and Building Materials 265:120303, DOI: https://doi.org/10.1016/j.conbuildmat.2020.120303
Zhang R, Wang X, Sun Y, Zhang J, Hu W, Du W, Chen G (2020b) Preparation and performance of ammonium-malic salts as shale swelling inhibitor and a mechanism study. Inorganic and Nano-Metal Chemistry 50(10):1027–1031, DOI: https://doi.org/10.1080/24701556.2020.1732418
Zhao Q, Li L, Li C, Li MD, Amini F, Zhang HZ (2014) Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease. Journal of Materials in Civil Engineering 26(12):04014094, DOI: https://doi.org/10.1061/(ASCE)MT.1943-5533.0001013
Acknowledgments
This study was supported by the National Natural Science Foundation of China (No. 52173248), the Transportation Department of Ningxia (No. 202000173) and the Science and Technology Department of Ningxia (No. 2020BFG02014).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wu, L., Miao, L., Kawasaki, S. et al. Effects of Reaction Conditions on EICP-Treated Desert Aeolian Sand. KSCE J Civ Eng 26, 2662–2674 (2022). https://doi.org/10.1007/s12205-022-1585-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12205-022-1585-0