Abstract
Fish products are essential sources of animal proteins and numerous nutrients required for healthy human nutrition worldwide. However, some types of low-priced fish may look very similar to some other expensive types, and usually, it is not easy to differentiate between them for inexperienced customers. Moreover, in some markets, adulterating such high-priced fish types through its substitution by cheaper ones or mixing with bacterially spoiled ones, mostly when sold as fish fillets, is sometimes common. Certainly, fish microbial contamination in open markets represents serious hazards for people’s public health. Accordingly, seeking easy and fast fish fraud detection methods and their microbial contamination disclosure is crucial. Currently, available techniques are costly, time-consuming, and requiring special laboratories. In the present work, laser-induced fluorescence (LIF), as a spectrochemical analytical technique and diffuse optical measurements, has been used to discriminate between fillets of low-priced Tilapia and expensive Nile Perch and disclose microbial contamination in any. The experimental data have been analyzed and evaluated using the principal component analysis (PCA), partial least square regression (PLSR), and receiver operatic characteristic (ROC) methods. The results demonstrated the high advantages of optical and spectrochemical techniques in the fast and accurate discrimination between the two fish species. Moreover, LIF spectral band obtained at 490 nm showed a difference in microbial load between both species.
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Arabi, D.S., Hamdy, O., Abdel-Salam, Z.A. et al. Utilization of Spectrochemical Analysis and Diffuse Optical Techniques to Reveal Adulteration of Alike Fish Species and Their Microbial Contamination. Food Anal. Methods 15, 1062–1073 (2022). https://doi.org/10.1007/s12161-021-02212-z
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DOI: https://doi.org/10.1007/s12161-021-02212-z