Abstract
Recently, consumption of collagen-based foodstuffs has grown significantly, especially collagen hydrolysate, used as a dietary supplement. Information available on the content of essential and toxic elements in these products, however, is incipient. This study provides a novel assessment of the mineral profile (Al, As, Ca, Cd, Co, Cr, Fe, K, Mg, Mn, Na, P, S, V, and Zn) of collagen hydrolysate and gelatin by inductively coupled plasma optical emission spectrometry (ICP OES) and mass spectrometry (ICP-MS). Thirty-two samples were digested with diluted HNO3 and H2O2, using an efficient microwave-assisted decomposition method (11.9% residual carbon content). The methodology was validated using certified reference materials and recovery tests, with agreement between the determined and certified/added concentration values (85–124%), and relative standard deviation of up to 18%. The limits of quantification ranged from 0.33 μg kg−1 (Cd) to 24 mg kg−1 (Na). The contributions of collagen hydrolysate to the Dietary Reference Intake values of the evaluated minerals were low, with the highest value being 13.5% for the Adequate Intake of Cr. The findings of Permitted Daily Exposure suggest that there is no risk of contamination with As and Cd by consuming this food supplement. Concentrations of Cr, Mn, and Zn reported on product labels agreed with experimental results. For Na, these values differed, indicating that a more rigorous quality control of collagen hydrolysate is necessary, in view of the health problems caused by excessive consumption of this element.
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Acknowledgements
Instrumental support provided by Agilent Technologies, Analítica, Milestone and Thermo Scientific is gratefully acknowledged. We are also thankful to Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA—Pecuária Sudeste) and Centro de Apoio à Pesquisa (CENAPESQ—UFRPE) for the use of physical facilities and analytical equipment, and to M.Sc. Fernanda Costa Pinheiro and M.Sc. Julymar Marcano de Higuera from the Group for Applied Instrumental Analysis, Federal University of São Carlos, for their assistance with ICP-MS measurements. The English text of this paper has been revised by Sidney Pratt, Canadian, MAT (The Johns Hopkins University), RSAdip—TESL (Cambridge University).
Funding
This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grant number 147727/2018-9), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) (grant number APQ-1017-1.06/15), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). We also would like to express our gratitude to the Instituto Nacional de Ciências e Tecnologias Analíticas Avançadas (INCTAA), CNPq (grant number 465768/2014-8), and FAPESP (grant number 2014/50951-4) for the financial support. Also, financial support for this was given by Núcleo de Química Analítica Avançada de Pernambuco (NUQAAPE/FACEPE) (grant number APQ-0346-1.06/14).
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Iago J. S. da Silva: conceptualization, methodology, validation, formal analysis, investigation, data curation, writing—original draft, visualization; Matheus L. de Mello: conceptualization, methodology, validation, investigation, writing—review and editing, visualization; André F. Lavorante: writing—review and editing, visualization; Ana P. S. Paim: conceptualization, resources, writing—review and editing, visualization, supervision; Joaquim A. Nóbrega: methodology, resources, writing—review and editing, visualization, supervision, project administration, funding acquisition; Maria J. da Silva: resources, writing—review and editing, visualization, supervision, project administration, funding acquisition.
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Iago J. S. da Silva declares that he has no conflict of interest. Matheus L. de Mello declares that he has no conflict of interest. André F. Lavorante declares that he has no conflict of interest. Ana P. S. Paim declares that she has no conflict of interest. Joaquim A. Nóbrega declares that he has no conflict of interest. Maria J. da Silva declares that she has no conflict of interest.
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da Silva, I.J.S., de Mello, M.L., Lavorante, A.F. et al. Evaluation of Mineral Profile and Dietary Reference Intake from Collagen by ICP-Based Techniques. Food Anal. Methods 14, 1860–1874 (2021). https://doi.org/10.1007/s12161-021-02015-2
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DOI: https://doi.org/10.1007/s12161-021-02015-2