Abstract
The salting-out induced liquid-liquid microextraction technique was properly evaluated for preservative determination in processed food samples. In the best conditions, 0.5 g sample and 4.5 mL acetate buffer, at pH 4.2, were extracted with 1 mL acetonitrile, followed by agitation, addition of 2 g MgSO4, a new agitation step, and centrifugation. Afterwards, the extract was diluted with ultrapure water and analyzed by high-performance liquid chromatography with ultraviolet detection. Correlation coefficients above 0.99, LOQs between 0.03 and 0.07 mg kg−1, and recoveries between 80 and 103%, with RSD ≤ 11%, were achieved. When applied to preservative determination in food samples, the method under evaluation provided similar results to those obtained by a previously validated one. Finally, this method is a good alternative to determining preservatives in food samples, with the advantages of being greener, cheaper, and faster than the official ones.
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Acknowledgments
The authors acknowledge the financial support granted by the Brazilian agencies FAPERGS (process number 19/2551-0001350-7), CNPq, and FINEP and fellowships granted by CAPES. The authors are thankful to the Centro Integrado de Análises for the chromatographic determinations. Part of this study was financially supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES)–Finance Code 001. E.G. Primel received a productivity research fellowship from the Brazilian Agency CNPq (DT 311694/2016-0).
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Jean Lucas de Oliveira Arias declares that he has no conflict of interest. Caroline Borges Rocha declares that she has no conflict of interest. Larine Kupski declares that she has no conflict of interest. Sergiane Caldas Barbosa declares that she has no conflict of interest. Ednei Gilberto Primel declares that he has no conflict of interest.
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de Oliveira Arias, J., Rocha, C.B., Kupski, L. et al. Salting-Out Induced Liquid-Liquid Microextraction: an Environmentally Friendly Approach to Preservative Determination in Food Samples. Food Anal. Methods 14, 1625–1636 (2021). https://doi.org/10.1007/s12161-021-01989-3
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DOI: https://doi.org/10.1007/s12161-021-01989-3