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Development of an Accelerated Solvent Extraction Approach for Quantitative Analysis of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Poultry Eggs

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Abstract

This manuscript describes a simple, reliable, and sensitive approach that utilizes high-performance liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-ESI/MS/MS) for the selective determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs (hen eggs, duck eggs, and goose eggs). Samples were extracted with methanol-ammonium hydroxide-ultrapure water (97:2:1, v/v) via accelerated solvent extraction (ASE) at 80 °C and 1500 psi and defatted with hexane saturated with acetonitrile. The experimental method uses ESI (+) and ESI (−) combination mode, full-scan qualitative analysis, and multiple reaction monitoring (MRM) combined with an internal standard for quantitative analysis. This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. The poultry eggs were spiked with the target compounds at four levels, and the average recoveries were all higher than 88.3%, and the relative standard deviations did not exceed 3.9%. The limits of detection and limits of quantification were 0.04–0.5 μg/kg and 0.1–1.5 μg/kg, respectively. The decision limits were 0.37–102 μg/kg, and the detection capabilities were 0.44–103 μg/kg. Finally, the new approach was successfully applied to the quantitative determination of these analytes in 120 commercial poultry eggs from local supermarkets.

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Funding

This research was financially supported by the China Agriculture Research System (CARS-41-G23), the Priority Academic Programme Development of Jiangsu Higher Education Institutions (PAPD), the Project of Drafting and Revision of the National Agriculture Industry Standard for Quality and Safety of Agricultural Products (181721301092371104), the Project of Jiangsu Agricultural Science and Technology Independent Innovation Fund (CX (18) 3022), the Yangzhou University High-end Talent Support Programme and the Yangzhou University International Academic Exchange Foundation.

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Authors and Affiliations

  1. College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

    Bo Wang & Xuezhong Liu

  2. Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou, 225009, China

    Bo Wang, Xia Zhao, Kaizhou Xie, Genxi Zhang, Tao Zhang & Xuezhong Liu

  3. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Xia Zhao, Kaizhou Xie, Genxi Zhang & Tao Zhang

  4. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China

    Xing Xie

Authors
  1. Bo Wang
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  2. Xia Zhao
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  3. Xing Xie
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  4. Kaizhou Xie
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  5. Genxi Zhang
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  6. Tao Zhang
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  7. Xuezhong Liu
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Correspondence to Kaizhou Xie.

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Bo Wang declares that he has no conflict of interest. Xia Zhao declares that he has no conflict of interest. Xing Xie declares that he has no conflict of interest. Kaizhou Xie declares that he has no conflict of interest. Genxi Zhang declares that he has no conflict of interest. Tao Zhang declares that he has no conflict of interest. Xuezhong Liu declares that he has no conflict of interest.

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Wang, B., Zhao, X., Xie, X. et al. Development of an Accelerated Solvent Extraction Approach for Quantitative Analysis of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Poultry Eggs. Food Anal. Methods 12, 1705–1714 (2019). https://doi.org/10.1007/s12161-019-01517-4

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