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Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid–Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology

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Abstract

A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid–liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box–Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1–7.8 ng/mL and limits of quantification (LOQs) of 3.5–26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.

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Acknowledgments

This work was supported by The National Natural Science Foundation of China (No. 31301595 and No. 21275089), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS1401) and Ph.D. research start-up funds of Qufu Normal University (bsqd 2012017).

Conflict of Interest

Hongliang Wu declares that he has no conflict of interest. Guoliang Li declares that he has no conflict of interest. Shucheng Liu declares that he has no conflict of interest. Zhongyin Ji declares that he has no conflict of interest. Qiulong Zhang declares that he has no conflict of interest. Na Hu declares that she has no conflict of interest. Yourui Suo declares that he has no conflict of interest. Jinmao You declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People’s Republic of China

    Hongliang Wu, Zhongyin Ji, Qiulong Zhang, Na Hu, Yourui Suo & Jinmao You

  2. Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People’s Republic of China

    Guoliang Li & Jinmao You

  3. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People’s Republic of China

    Hongliang Wu, Zhongyin Ji, Qiulong Zhang & Na Hu

  4. College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang, People’s Republic of China

    Guoliang Li & Shucheng Liu

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  1. Hongliang Wu
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Correspondence to Guoliang Li or Jinmao You.

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Hongliang Wu and Guoliang Li contributed equally to this work.

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Wu, H., Li, G., Liu, S. et al. Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid–Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology. Food Anal. Methods 8, 685–695 (2015). https://doi.org/10.1007/s12161-014-9943-2

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  • DOI: https://doi.org/10.1007/s12161-014-9943-2

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