Abstract
In this study, a simple and highly sensitive electroanalytical method for the determination of thiabendazole (TBZ) was developed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) modified glassy carbon electrode (MWCNT-COOH/GCE) in phosphate buffer solution (pH = 6.0). MWCNT-COOH/GCE exhibits an enhanced effectiveness for the redox of TBZ. This constructed biosensor exhibited two linear relationships with TBZ concentration range from 5.00 × 10−7 to 4.95 × 10−6 mol/L and 4.95 × 10−6 to 9.80 × 10−5 mol/L, respectively. The detection limit was 3.0 × 10−7 mol/L (S/N = 3) and the relative standard deviation (R.S.D.) was lower than 4.2 % (n = 6). The proposed method was successfully applied to determine TBZ in real samples and the results were satisfactory. The MWCNT-COOH/GCE electrode showed good reproducibility, stability, and anti-interference. The electrochemistry method was also described for the evaluation of TBZ and human serum albumin (HSA) interaction. In the presence of HSA, the peak currents of TBZ decreased linearly due to the formation of a kind of electro-inactive complex. The binding constant between TBZ and HSA was obtained by DPV.
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Acknowledgments
This project was supported by the National Nature Science Foundation of China (NSFC51178212), the National Science Foundation of Liaoning Province, China (No.201102082), Liaoning Provincial Department of Education Innovation Team Projects (LT2012001), the Shenyang Science and Technology Plan (F12-277-1-69), and the Foundation of 211 Project for Innovative Talent Training, Liaoning University. The authors also thank their colleagues and other students who participated in this work.
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Jichun Yang declares that he has no conflict of interest. Danfeng Zhang declares that she has no conflict of interest. Lingling Wang declares that she has no conflict of interest. Na Long declares that she has no conflict of interest. Minhui Zhang declares that she has no conflict of interest. Lei Zhang declares that she has no conflict of interest.
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Yang, J., Zhang, D., Wang, L. et al. An Electrochemical Method for High Sensitive Detection of Thiabendazole and Its Interaction with Human Serum Albumin. Food Anal. Methods 8, 507–514 (2015). https://doi.org/10.1007/s12161-014-9919-2
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DOI: https://doi.org/10.1007/s12161-014-9919-2