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Simultaneous Quantitation of FFA, MAG, DAG, and TAG in Enzymatically Modified Vegetable Oils and Fats

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Abstract

A novel and easy-to-implement high temperature gas chromatographic procedure for the simultaneous quantitation of free fatty acids (FFA), monoacylglycerols (MAG), diacylglycerols (DAG), and triacylglycerols (TAG) for products arising from fats and oils modification processes has been developed. The method involves silylation in the presence of pyridine at room temperature, standard injection system, and FID detection. Species were separated by carbon number and degree of unsaturation; thus, a total of 53 individual compounds could be quantified. An identification plot for unavailable triacylglycerol standard species relating relative retention time with the number of double bonds was built. Calibration curves for the quantitation of by-products (FFA, MAG, and DAG) and a calibration surface for TAG were constructed using standard solutions. Multiple internal standards were used, achieving good repeatability. The method was tested over a wide range of fatty acid unsaturation level (0 to 3 double bonds) and chain length (C14 to C18). The results achieved in real samples were in agreement with those obtained by fatty acid methyl esters (FAME) analysis and HPLC-RI. The method developed is a rapid and reliable technique to quantify FFA, MAG, DAG, and TAG in substrates and products of modification, ideal to monitor the degree of conversion in fats and oils enzymatic esterification, among others.

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Acknowledgments

This work received financial support from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), and the Universidad Nacional del Sur (UNS) of Argentina.

Conflict of Interest

Consuelo Pacheco declares that she has no conflict of interest. Camila Palla declares that she has no conflict of interest. Guillermo H. Crapiste declares that he has no conflict of interest. María E. Carrín declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. PLAPIQUI (Universidad Nacional del Sur—CONICET), Camino La Carrindanga Km 7, 8000, Bahía Blanca, Argentina

    Consuelo Pacheco, Camila Palla, Guillermo H. Crapiste & María E. Carrín

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  1. Consuelo Pacheco
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  2. Camila Palla
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  3. Guillermo H. Crapiste
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  4. María E. Carrín
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Correspondence to María E. Carrín.

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Pacheco, C., Palla, C., Crapiste, G.H. et al. Simultaneous Quantitation of FFA, MAG, DAG, and TAG in Enzymatically Modified Vegetable Oils and Fats. Food Anal. Methods 7, 2013–2022 (2014). https://doi.org/10.1007/s12161-014-9830-x

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  • DOI: https://doi.org/10.1007/s12161-014-9830-x

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