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A Specific and High-Throughput Fluorescence Polarization Immunoassay for Surveillance Screening of Clinafloxacin in Milk

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Abstract

In this study, antibody specific to clinafloxacin (CLI) was generated for the first time, and a rapid and specific surveillance screening method, fluorescence polarization immunoassay (FPIA), was developed for the detection of CLI residue in milk. The developed FPIA showed a working range from 0.8 to 787.3 μg/L with the IC50 of 25.6 μg/L. The cross-reactivity to other fluoroquinolone drugs was negligible, which demonstrated high specificity. Good recoveries, ranged from 97.9 to 112.7 %, in spiked milk samples were obtained. The proposed FPIA was also validated by high performance liquid chromatography as a reference method, and the two methods yielded concordant results. The investigation indicated that the proposed FPIA is suitable for surveillance screening purposes to detect CLI residue in milk.

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Acknowledgments

This work was supported by Natural Science Foundation of China (U1301214), Guangdong Natural Science Foundation (S2013030013338), the Ph.D. Programs Foundation of Ministry of Education of China (20114404130002), National Department Public Benefit Research Foundation (201003008-08), and Guangdong Higher Education Project for Transformation of Scientific and Technological Achievements (cgzhzd0808).

Conflict of Interest

Jiahong Chen declares that she has no conflict of interest. Qiang Wang declares that he has no conflict of interest. Shuwei Lv declares that he has no conflict of interest. Zhenlin Xu declares that he has no conflict of interest. Jinyi Yang declares that he has no conflict of interest. Yudong Shen declares that he has no conflict of interest. Hong Wang declares that she has no conflict of interest. Yuanming Sun declares that he has no conflict of interest. Hongtao Lei declares that he has no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Preservation (Guangzhou), Ministry of Agriculture/Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China

    Jiahong Chen, Shuwei Lv, Zhenlin Xu, Jinyi Yang, Yudong Shen, Hong Wang, Yuanming Sun & Hongtao Lei

  2. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China

    Qiang Wang

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  1. Jiahong Chen
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  2. Shuwei Lv
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  3. Qiang Wang
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  4. Zhenlin Xu
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  5. Jinyi Yang
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  6. Yudong Shen
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  7. Hong Wang
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  8. Yuanming Sun
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  9. Hongtao Lei
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Corresponding author

Correspondence to Hongtao Lei.

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Fig. S1

ESI-MS of the tracer CLI-FITC (R f  = 0.3) (DOC 111 kb)

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Chen, J., Lv, S., Wang, Q. et al. A Specific and High-Throughput Fluorescence Polarization Immunoassay for Surveillance Screening of Clinafloxacin in Milk. Food Anal. Methods 8, 1468–1476 (2015). https://doi.org/10.1007/s12161-014-0033-2

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  • DOI: https://doi.org/10.1007/s12161-014-0033-2

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