Abstract
Liquid chromatography Orbitrap mass spectrometry method for quantification of biological aminothiols (cysteine, homocysteine, and glutathione) in cereal products has been developed. The assay is based on preliminary derivatization with N-(2-acridonyl)maleimide and high resolution accurate mass spectrometry with utilization of dl-Homocystine-3,3,3′,3′,4,4,4′,4′-d8 (homocystine-d8) as internal standard. The limits of quantification for homocysteine, cysteine, and glutathione are 19.44, 40.78, and 338.93 pg, respectively, per 10 μl injection. Intra- and inter-day precision expressed as relative standard deviations are in the range of 1.76 to 2.94 % and 1.06 to 4.13 %, respectively. The average recoveries were 98 % for Hcy, 87 % for Cys, and 92 % for GSH. Wheat, maize, and bakery products with different origin were analyzed. The content of Hcy in the investigated samples was found to be in range of 9–436 μg/100 g, Cys in range of 29–6,895 μg/100 g and GSH in range of 259–14,795 μg/100 g.
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Acknowledgment
This research was supported by National Science Fund of Bulgaria (grand DUNK 01/2 2009) and by the Counsel of Medical Science at Medical University Sofia (Grands 21-D 2011).
Conflict of Interest
Silviya Ruseva has received a personal research grant (Grants 21-D 2011) from Counsel of Medical Science at Medical University Sofia. Anelia Vasileva declares that she has no conflict of interest. Doroteya Aleksandrova declares that she has no conflict of interest. Valentin Lozanov declares that he has no conflict of interest. Vanio Mitev declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.
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Ruseva, S., Vasileva, A., Aleksandrova, D. et al. Liquid Chromatography Orbitrap Mass Spectrometry Method for Analysis of Homocysteine and Related Aminothiols in Cereal Products. Food Anal. Methods 7, 865–871 (2014). https://doi.org/10.1007/s12161-013-9693-6
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DOI: https://doi.org/10.1007/s12161-013-9693-6