Abstract
A sensitive and reliable method for the simultaneous determination of triclabendazole, its main metabolites (triclabendazole sulfone and triclabendazole sulfoxide) and a marker residue (ketotriclabendazole) in edible ruminant fat tissues is developed and validated. Analytes are extracted from fat tissues with acetonitrile, and crude extracts are subjected to liquid–liquid extraction with n-hexane. Chromatographic separation is performed on a C18 reversed-phase column with gradient elution. Analytes are detected by tandem quadrupole mass spectrometry after positive electrospray ionization by multiple reaction monitoring. Relative recoveries from spiked samples range from 86.1% to 114.3%, with relative standard deviations generally below 13.8%. Limits of detection and quantification for analytes are within 0.125–1.25 μg/kg and 0.5–5 μg/kg, respectively. The method proposed in this study was successfully applied to real sample testing.
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Acknowledgments
The authors would like to acknowledge the Key Laboratory of Animal Parasitology of the Ministry of Agriculture for providing the laboratory equipment used in this study. This work was funded by the Ministry of Agriculture of the People’s Republic of China.
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Cai, C., Xue, F., Wang, Z. et al. Simultaneous Determination of Triclabendazole and its Metabolites in Bovine and Goat Fat Tissues by Liquid Chromatography–Tandem Mass Spectrometry. Food Anal. Methods 5, 1260–1266 (2012). https://doi.org/10.1007/s12161-012-9372-z
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DOI: https://doi.org/10.1007/s12161-012-9372-z