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Long non-coding RNA PVT1 facilitates cell migration and invasion by regulating miR-148a-3p and ROCK1 in breast cancer

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Abstract

Purpose

Breast cancer (BC) is one of the most common malignant tumors for women. The role and potential mechanisms of long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) were explored in BC cell migration and invasion.

Methods

PVT1, miR-148a-3p and Rho‑associated, coiled‑coil containing protein kinase 1 (ROCK1) mRNA expressions were detected using real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The ROCK1 protein expression was detected by Western blotting. The relationship of PVT1, miR-148a-3p and ROCK1 was analyzed by Dual Luciferase activity, RNA immunoprecipitation (RIP) and Spearman correlation analysis. Cell invasion and migration were detected by Transwell assay.

Results

Upregulation of PVT1 and ROCK1, and downregulation of miR-148a-3p were observed in BC tissues and cell lines. According to the analysis of Dual Luciferase activity, RIP and Spearman correlation analysis, miR-148a-3p directly binds to PVT1, and ROCK1 is a target of miR-148a-3p. In addition, PVT1 regulated the cells migration and invasion by regulating miR-148a-3p and ROCK1 expression.

Conclusion

These data demonstrated that PVT1 was upregulated and facilitated to the cell migration and invasion of BC by the regulation of miR-148a-3p and ROCK1, indicating that PVT1 may be a potential biomarker of BC diagnosis and treatment.

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Author information

Authors and Affiliations

  1. Emergency Department, Weifang People’s Hospital, Weifang, 261041, China

    H.L Li

  2. Breast Diagnosis and Treatment Center, Affiliated Qingdao Central Hospital, Qingdao University, Qingdao, 266042, China

    C.P Wang

  3. Radiology Department, Affiliated Qingdao Central Hospital, Qingdao University, Qingdao, 266042, China

    Y. Zhang

  4. Department of Neurology, the People’s Hospital of Zhangqiu Area, Jinan, 250200, China

    J.X Du

  5. Department of Health Examination, the People’s Hospital of Zhangqiu Area, Jinan, 250200, China

    L.H Han

  6. Department of Oncology, Jinan Central Hospital, 105 Jiefang Road, Jinan, 250013, Shandong, China

    H.Y Yang

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  1. H.L Li
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  2. C.P Wang
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  3. Y. Zhang
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  4. J.X Du
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  5. L.H Han
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  6. H.Y Yang
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Contributions

HLL designed the study and drafted the manuscript. CPW and YZ were responsible for the collection and analysis of the experimental data. JXD, LHH and HYY revised the manuscript critically for important intellectual content. All authors read and approved the final manuscript.

Corresponding author

Correspondence to H.Y Yang.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by Ethical Committee of Jinan Central Hospital and conducted in Accordance with the Ethical Standards.

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Li, H., Wang, C., Zhang, Y. et al. Long non-coding RNA PVT1 facilitates cell migration and invasion by regulating miR-148a-3p and ROCK1 in breast cancer. Clin Transl Oncol 24, 882–891 (2022). https://doi.org/10.1007/s12094-021-02736-0

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  • DOI: https://doi.org/10.1007/s12094-021-02736-0

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