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A novel peptide (Thx) homing to non-small cell lung cancer identified by ex vivo phage display

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Abstract

Aim

To identify linear peptide homing to non-small cell lung cancer (NSCLC) tumor cells using ex vivo phage display method.

Materials and methods

Twenty-six clinical patient samples were used to identify linear homing peptide, which was exposed to NSCLC cell cultures and control cell lines to determine cell binding affinity and cell localization. Also, ex vivo biodistribution was analyzed using tumor-bearing mice.

Results

The panning yielded peptide enrichment with a core motif A/SRXPXXX. Based on this, an amino acid sequence, ARRPKLD, was selected for characterization and named Thx-peptide. The in vitro binding properties of Thx-peptide demonstrated selectivity toward NSCLC. Internalization assays showed that Thx-Alexa and fluorescein conjugates were located in a subset of perinuclearly located lysosomes of tumor cells. Thx-peptide appeared with fluorescein-labeled peptide and peptide-DTPA-chelator complex in adenocarcinoma xenografts in mice.

Conclusion

Thx shows promise for targeted imaging and drug delivery.

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Conflict of interest

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Authors and Affiliations

  1. Karyon-CTT Ltd., Helsinki, Finland

    A. Koivistoinen, K. Punakivi & M. Bergman

  2. Division of General Thoracic and Esophageal Surgery, Department of Cardiothoracic Surgery, Helsinki University Central Hospital, Haartmaninkatu 4, PO Box 340, 00290, Helsinki, Finland

    I. I. K. Ilonen, J. V. Räsänen, E. I. Sihvo & J. A. Salo

  3. Department of Pathology, Helsinki University Central Hospital, Helsinki, Finland

    H. Helin

Authors
  1. A. Koivistoinen
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  2. I. I. K. Ilonen
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  3. K. Punakivi
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  4. J. V. Räsänen
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  5. H. Helin
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  6. E. I. Sihvo
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  7. M. Bergman
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  8. J. A. Salo
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Correspondence to J. A. Salo.

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Koivistoinen, A., Ilonen, I.I.K., Punakivi, K. et al. A novel peptide (Thx) homing to non-small cell lung cancer identified by ex vivo phage display. Clin Transl Oncol 15, 492–498 (2013). https://doi.org/10.1007/s12094-012-0959-z

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  • DOI: https://doi.org/10.1007/s12094-012-0959-z

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