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Phomopsis tersa as Inhibitor of Quorum Sensing System and Biofilm Forming Ability of Pseudomonas aeruginosa

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Abstract

Endophytic fungi provide rich reservoir for novel antimicrobial compounds. An endophytic fungus, from Carica papaya plant identified as Phomopsis tersa, was investigated for attenuating the quorum sensing mediated pathogenicity of Pseudomonas aeruginosa PAO1. Crude extract of P. tersa was found to reduce the production of redox-active pigments—pyocyanin and pyoverdine in P. aeruginosa PAO1 by 92.46% and 71.55%, respectively at sub-MIC concentration of 900 μg/mL. In addition, the crude extract was also able to inhibit the expression of virulence factors involved in biofilm formation: exopolysaccharide (72.21%) and alginate (72.50%). Secretion of cell-lytic enzymes was also found to be reduced: chitinase by 79.73% and elastase by 74.30%. 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione identified from GC-MS analysis, displayed favorable molecular interactions with P. aeruginosa transcriptional regulators, LasR and RhlR with good docking scores of − 6.873 kJ/mol and − 6.257 kJ/mol, respectively. The study thus reveals the potential use of P. tersa for discovering drugs against infectious pathogens.

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Acknowledgements

We thank Fungal Biotechnology Laboratory, Department of Biotechnology, Pondicherry University for isolating the fungi from Carica papaya. Dr. Busi Siddhardha is thankful to the start-up research grant from the Department of Science and Technology – The Science & Engineering Research Board [SB/YS/LS-32/2014] for funding the project work. This work was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2019H1D3A2A01060226) to work at Konkuk University (VCK). This research was supported by Basic Science Research Program through the NRF funded by the Ministry of Science, ICT & Future Planning (2013M3A6A8073184) (JKL).

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Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India

    Himani Meena & Busi Siddhardha

  2. Fungal Biotechnology Lab, Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India

    Rashmi Mishra & V. Venkateswara Sarma

  3. Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, 605014, India

    Sampathkumar Ranganathan & Dinakara Rao Ampasala

  4. Department of Chemical Engineering, Konkuk University, Seoul, 05029, Republic of Korea

    Vipin Chandra Kalia & Jung-Kul Lee

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  1. Himani Meena
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Correspondence to Vipin Chandra Kalia, Jung-Kul Lee or Busi Siddhardha.

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Meena, H., Mishra, R., Ranganathan, S. et al. Phomopsis tersa as Inhibitor of Quorum Sensing System and Biofilm Forming Ability of Pseudomonas aeruginosa. Indian J Microbiol 60, 70–77 (2020). https://doi.org/10.1007/s12088-019-00840-y

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