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Probiotic Strains Influence on Infant Microbiota in the In Vitro Colonic Fermentation Model GIS1

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Abstract

The main goal of our study was to evaluate the effect of the individual administration of five lyophilized lactic acid bacteria strains (Lactobacillus fermentum 428ST, Lactobacillus rhamnosus E4.2, Lactobacillus plantarum FCA3, Lactobacillus sp. 34.1, Weissella paramesenteroides FT1a) against the in vitro simulated microbiota of the human colon using the GIS1 system. The influence on the metabolic activity was also assessed by quantitative determination of proteins and polysaccharides at each segment of human colon. The obtained results indicated that the lactic acid bacteria L. rhamnosus E4.2 and W. paramesenteroides FTa1 had better efficiency in synthesising exopolysaccharides and also a better probiotic potential and therefore could be recommended for use in probiotics products or food industry.

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Acknowledgments

This research was supported by the National Centre for Program Management PN-II-PT-PCCA-2011-3.1-0969/2012 coordinated by Romanian Agency for Scientific Research.

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Authors and Affiliations

  1. Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031, Bucharest, Romania

    Veronica Ionela Moroeanu, Gabriela Paun, Elena Neagu, Oana Rodica Ungureanu, Sandra A. V. Eremia & Gabriel-Lucian Radu

  2. Department of Industrial Biotechnology, Faculty of Biotechnology, University of Agronomical Sciences and Veterinary Medicine Bucharest, 59 Marasti blvd, 1 district, 011464, Bucharest, Romania

    Emanuel Vamanu

  3. Faculty of Biology, MICROGEN - Center for Research in Microbiology, Genetics and Biotechnology, University of Bucharest, 1-3 Portocalilor Str., 5 district, 060101, Bucharest, Romania

    Robertina Ionescu & Diana Roxana Pelinescu

Authors
  1. Veronica Ionela Moroeanu
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  2. Emanuel Vamanu
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  3. Gabriela Paun
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  4. Elena Neagu
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  5. Oana Rodica Ungureanu
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  6. Sandra A. V. Eremia
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  7. Gabriel-Lucian Radu
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  8. Robertina Ionescu
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  9. Diana Roxana Pelinescu
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Correspondence to Emanuel Vamanu.

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Moroeanu, V.I., Vamanu, E., Paun, G. et al. Probiotic Strains Influence on Infant Microbiota in the In Vitro Colonic Fermentation Model GIS1. Indian J Microbiol 55, 423–429 (2015). https://doi.org/10.1007/s12088-015-0542-8

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  • DOI: https://doi.org/10.1007/s12088-015-0542-8

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