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Interaction of Azospirillum brasilense and Glomus intrarradix in Sugar Cane Roots

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Abstract

Fifteen-day-old variety NA 56-79 sugar cane seedlings were inoculated with Azospirillum brasilense and Glomus intrarradix. This article aims at examining changes in sugar cane root seedlings inoculated with Glomus intrarradix and Azospirillum brasilense, the increase in microbial biomass and the acetylene reduction process as well. The internal root colonization was studied 20 days after inoculation using scanning and a transmission electron microscope. Both microorganisms entered the sugar cane root through the emergent lateral roots. The microorganisms were capable of coexisting both intra and intercellularly, producing changes in the cell wall, thus allowing colonization and interaction between the organisms. These changes increased the number of microorganisms inside the root as well as acetylene nitrogen reduction. Sugar cane plant biomass increased with joint-inoculation. The number of endophytic microorganisms and nitrogen fixing activity increased when they were colonized by Azospirillum and Glomus together.

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Authors and Affiliations

  1. Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, Av. Roca 1900, 4000, Tucumán, Argentina

    Carlos H. Bellone & Carrizo de Bellone Silvia

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  1. Carlos H. Bellone
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  2. Carrizo de Bellone Silvia
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Correspondence to Carlos H. Bellone.

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Bellone, C.H., de Bellone Silvia, C. Interaction of Azospirillum brasilense and Glomus intrarradix in Sugar Cane Roots. Indian J Microbiol 52, 70–75 (2012). https://doi.org/10.1007/s12088-011-0208-0

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  • DOI: https://doi.org/10.1007/s12088-011-0208-0

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