Abstract
Odorant binding proteins are expressed in the olfactory epithelium of many mammalian species and are thought to assist in the uptake and transfer of odorants from the gas phase to the odor receptors. Various mechanisms have been proposed to explain how mass transfer occurs. Most experimental work has focused on the binding of ligands to odorant binding proteins or to the olfactory receptors under steady-state conditions, whereas the situation in vivo is dynamic due to the tidal flow of air through the nose and the mass transfer in and out of the olfactory epithelium. Some preliminary dynamic data have been obtained from an in vitro system, and this has been used, along with other published data, to test some of the proposed mechanisms for odorant binding protein (OBP). An assessment of the hypothetical mechanisms has been made to examine how well the mechanisms fit with the published experimental data. A new hypothesis is proposed where OBP acts to prolong the odor signal and increases the signal output of the olfactory system.
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References
Briand L, Nespoulous C, Perez V, Remy JJ, Huet JC, Pernollet JC (2000) Ligand-binding properties and structural characterization of a novel rat odorant-binding protein variant. Eur J Biochem 267(10):3079–3089
Crank J, McFarlane NR, Newby JC, Paterson GD, Pedley JB (1981) Diffusion processes in environmental systems. Macmillan, London
Cunningham AB, Ross RJ (2007) The Biofilms Hypertextbook. Retrieved 23 December 2007
deRoos KB, Wolswinkel K (1994) Non-equilibrium partition model for predicting flavour release in the mouth. In: Maarse H, Van der Heij DG (eds) Trends in flavour research. Elsevier Science, Amsterdam, pp 15–32
Farbman AI (2002) Olfactory organs of vertebrates. In: Finger TE, Silver WL, Restrepo D (eds) The neurobiology of taste and smell, 2nd edn. Wiley-Liss, New York
Firestein S, Picco C, Menini A (1993) The relation between stimulus and response in olfactory receptor-cells of the tiger salamander. J Physiol (Lond) 468:1–10
Flower DR (1996) The lipocalin protein family: Structure and function. Biochem J 318:1–14
Hahn I, Scherer PW, Mozell MM (1994) A mass-transport model of olfaction. J Theor Biol 167(2):115–128
Hummel T, Kobal G (2002) Olfactory event-related potentials. In: Simon SA, Nicolelis MAL (eds) Methods in chemosensory research. CRC, Boca Raton
Hwang PM, Pevsner J, Sklar PB, Venable JC, Snyder SH (1988) Localization of rat odorant-binding protein to the lateral nasal gland suggests an odorant transport function. Chem Senses 13(4):699–699
Laing DG, Macleod P (1992) Reaction time for the recognition of odor quality. Chem Senses 17(3):337–346
Laing DG, Eddy A, Francis GW, Stephens L (1994) Evidence for the temporal processing of odor mixtures in humans. Brain Res 651(1–2):317–328
Linforth RST, Taylor AJ (2003) Direct mass spectrometry of complex volatile and non-volatile flavour mixtures. Int J Mass Spectrom 223–224:179–191
Lobel D, Jacob M, Volkner M, Breer H (2002) Odorants of different chemical classes interact with distinct odorant binding protein subtypes. Chem Senses 27(1):39–44
Mair RG, Gesteland RC, Blank DL (1982) Changes in morphology and physiology of olfactory receptor cilia during development. Neuroscience 7(12):3091–3103
Marin M, Baek I, Taylor AJ (1999) Flavour release from aqueous solutions under dynamic headspace dilution conditions. J Agric Food Chem 47:4750–4755
Marin M, Baek I, Taylor AJ (2000) Flavor release as a unit operation: a mass transfer approach. In: Roberts DD, Taylor AJ (eds) Flavor release, vol 763. American Chemical Society, Washington, DC, pp 153–164
Matarazzo V, Zsurger N, Guillemot JC, Clot-Faybesse O, Botto JM, Dal Farra C et al (2002) Porcine odorant-binding protein selectively binds to a human olfactory receptor. Chem Senses 27(8):691–701
Menco BPM, Farbman AI (1992) Ultrastructural evidence for multiple mucous domains in frog olfactory epithelium. Cell Tissue Res 270(1):47–56
Nespoulous C, Briand L, Delage MM, Tran V, Pernollet JC (2004) Odorant binding and conformational changes of a rat odorant-binding protein. Chem Senses 29(3):189–198
Pernollet J-C, Briand L (2004) Structural recognition between odorants, olfactory-binding proteins and olfactory receptors—first events in odour coding. In: Taylor AJ, Roberts DD (eds) Flavor perception. Blackwell, Oxford, pp 86–150
Pevsner J, Snyder SH (1990) Odorant-binding protein—odorant transport function in the vertebrate nasal epithelium. Chem Senses 15(2):217–222
Sjodal M, Edlund T, Gunhaga L (2007) Time of exposure to bmp signals plays a key role in the specification of the olfactory and lens placodes ex vivo. Dev Cell 13:141–149
Steinbrecht RA (1998) Odorant-binding proteins: expression and function. In: Olfaction and taste XII. New York Academy of Sciences, New York, vol 855, pp 323–332
Stewart PS (1998) A review of experimental measurements of effective diffusive permeabilities and effective diffusion coefficients in biofilms. Biotechnol Bioeng 59(3):261–272
Taylor AJ (1998) Physical chemistry of flavour. Int J Food Sci Technol 33(1):53–62
Taylor AJ (2002) Release and transport of flavours in vivo: physico chemical, physiological and perceptual considerations. Comprehensive Reviews in Food Safety and Food Science 1:45–57
Weisstein EW (2002) CRC concise encyclopedia of mathematics, 2nd edn. CRC, Boca Raton
Acknowledgments
We thank Loic Briand and Jean-Claude Pernollet (INRA Jouy-en-Josas, France) for generously supplying OBP and helping in setting up OBP production in our laboratory as well as discussing the merits of the different OBP binding assays. An anonymous reviewer helpfully suggested the idea of ciliary action and its potential effect on mass transfer. The UK research council, BBSRC, provided funding for the practical part of the project.
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Taylor, A.J., Cook, D.J. & Scott, D.J. Role of Odorant Binding Proteins: Comparing Hypothetical Mechanisms with Experimental Data. Chem. Percept. 1, 153–162 (2008). https://doi.org/10.1007/s12078-008-9016-2
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DOI: https://doi.org/10.1007/s12078-008-9016-2