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Palladium(II) complex bearing benzothiazole based O,N,S donor pincer ligand: Study of in-vitro cytotoxicity, interaction with CT-DNA and BSA protein

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Abstract

A new palladium(II) complex, [Pd(LSEt)Cl] (C1) with benzothiazole based ONS donor pincer ligand (HLSEt) was synthesized (where, HLSEt = 2-(benzothiazol-2-yl)-6-(((2-(ethylthio)phenyl)imino)methyl)phenol). Interaction of C1 with CT DNA was investigated, and its binding constant was found to be 4.0×105 M−1. The proficiency of ethidium bromide (EB) displacement from its EB-CTDNA complex by C1 was performed by the fluorescence quenching method, and Stern-Volmer quenching constant (Ksv) was found to be 4.3×105 M−1. Similarly, the interaction of C1 with BSA protein was investigated by UV-Vis and fluorescence methods. The apparent association constant (Ka) and Ksv were determined (Ka = 2.8×104 M−1 and Ksv = 5.5×104 M−1). In vitro cytotoxicity of the complex, [Pd(LSEt)Cl] (C1), towards human gastric cancer cell lines (AGS) was assessed by the MTT assay method. The half maximal inhibitory concentration (IC50) of C1 (9.55 ± 1.23 µM) towards AGS cancer lines was found to be lower than cisplatin (23.13 ± 1.03 µM).

Graphical abstract

Herein, new palladium(II) complex, [Pd(LSEt)Cl] (C1) with benzothiazole-based ONS donor pincer ligand (HLSEt) was synthesized and characterized by several spectroscopic techniques. Interaction of C1 with CT DNA and BSA protein was investigated by UV-Vis and fluorescence methods. In vitro cytotoxicity of the complex toward human gastric cancer cell lines (AGS) was evaluated by the MTT assay method. The half maximal inhibitory concentration (IC50) of the palladium(II) complex (9.55±1.23 µM) was found to be less compared to cisplatin (23.13±1.03 µM) towards AGS cancer lines.

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Acknowledgment

The work is financially supported by Science and Engineering Research Board (SERB), New Delhi, India (No. EEQ/2018/000266). UGC, New Delhi, India, is gratefully acknowledged for fellowship.

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  1. Inorganic Chemistry Section, Department of Chemistry, Jadavpur University, Kolkata, 700032, India

    RAHUL NASKAR, SUBRATA MANDAL, SUBRATA JANA & TAPAN KUMAR MONDAL

  2. Department of Signal Transduction and Biogenic Amines (STBA), Chittaranjan National Cancer Institute, Kolkata, 700026, India

    PARAMITA GHOSH & NABENDU MURMU

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NASKAR, R., GHOSH, P., MANDAL, S. et al. Palladium(II) complex bearing benzothiazole based O,N,S donor pincer ligand: Study of in-vitro cytotoxicity, interaction with CT-DNA and BSA protein. J Chem Sci 134, 103 (2022). https://doi.org/10.1007/s12039-022-02101-w

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