Skip to main content
SpringerLink
Log in

Biological synthesis and characterization of silver nanoparticles using Eclipta alba leaf extract and evaluation of its cytotoxic and antimicrobial potential

  • Published:
Bulletin of Materials Science Aims and scope Submit manuscript

Abstract

With increasing global competitions there is a growing need to develop environmentally benevolent nanoparticles without the use of toxic chemicals. The biosynthesis of silver nanoparticles (AgNPs) using plant extracts became one of the potential areas of research. The bioreduction of metal ion is quite rapid, readily perform at room temperature and easily scale up. The present study describes a rapid and eco-friendly synthesis of AgNPs using Eclipta alba plant extract in a single pot process. The efficiency and the influence of various process variables in the biosynthesis of AgNPs analysed include redundant concentration, temperature and time. AgNPs were rapidly synthesized using aqueous leaf extract of E. alba and was observed when the medium turned to brown colour with the addition of silver ion. Biosynthesized AgNPs were characterized by the help of UV–visible spectroscopy for their stability and physicochemical parameters were studied by dynamic light scattering, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy. The obtained results confirmed that recorded UV spectra show the characteristic surface plasmon resonance band for AgNPs in the range of 400–440 nm and physiochemical structural analysis shown that obtained AgNPs were crystalline in nature. Further, cytotoxic and antimicrobial activities of biosynthesized AgNPs against RAW 254.7, MCF-7 and Caco-2 cells as well as Gram positive and Gram negative bacteria were assessed. In-vitro cytotoxicity activity of characterized AgNPs against tested cell lines showed significant anti-cell-proliferation effect in nanomolar concentrations. The antibacterial activity of synthesized AgNPs showed effective inhibitory activity against human pathogens, including, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Thus, the significant outcome of this study would help to formulate value added herbal-based nano-materials in biomedical and nanotechnology industries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10

Similar content being viewed by others

References

  1. Robert T, Morrison Robert N, Boyd and Robert K 1992 Organic chemistry, 6th edition

  2. Catauro M, Raucci M G, de Gaetano F and Marotta A 2004 J. Mater. Sci: Mater. Med. 15 831

  3. Crabtree J H, Burchette R J, Siddiqi R A, Huen I T, Handott L L and Fishman A 2003 Peritoneal Dialysis Int. 23 368

  4. Krolikowska A, Kudelski A, Michota A and Bukowska J 2003 Surf. Sci. Rep. 532 227

  5. Zhao G J and Stevens S E 1998 Biometals 11 27

  6. Daniel M C and Astruc D 2004 Chem. Rev. 104 293

  7. Kato H 2011 Nat. Nanotechnol. 6 139

  8. Mittal A K, Chisti Y and Banerjee U C 2013 Biotechnol. Adv. 31 346

  9. Singhal G R, Bhavesh K, Kasariya A R, Sharma and Singh R P 2011 J. Nanopart. Res. 13 2981

  10. Kim J S, Kuk E, Yu K N, Kim J H, Park S J, Lee H J, Kim S H, Park Y K, Park Y H, Hwang C Y, Kim Y K, Lee Y S, Jeong D H and Cho M H 2007 Nanomedicine: Nanotechnol. Biol. Med. 3 95

  11. Rai M, Yadav A and Gade A 2009 Biotechnol. Adv. 27 76

  12. Chen S, Wu G and Zeng H 2005 Carbohydr. Polym. 60 33

  13. Castellano J J, Shafii S M, Ko F, Donate G, Wright T E, Mannari R J, Payne W G, Smith D J and Robson M C 2007 Int. Wound J. 4 114

  14. Jung R, Kim Y, Kim H S and Jin H J 2009 J. Biomater. Sci. Polym. Ed. 20 311

  15. Yu D G 2007 Colloids Surf. B: Biointerfaces 59 171

  16. Liu Y C and Lin L N 2004 Electrochem. Commun. 6 1163

  17. Mallick K, Witcombb M J and Scurrella M S 2005 Mater. Chem. Phys. 90 221

  18. Nayak R R, Pradhan N, Behera D, Pradhan K M, Mishra S, Sukla L B and Mishra B K 2011 J. Nanopart. Res. 13 3129

  19. Pattabi M and Uchil J 2000 Sol. Energy Mater. Sol. Cell 63 309

  20. Lin C Y C, Jacob D J, Munger J W and Fiore A M 2000 Geophys. Res. Lett. 27 3465

  21. Jae Y S and Beom S K 2009 Bioprocess. Biosyst. Eng. 32 79

  22. Hutchison J E 2008 ACS Nano. 2 395

  23. Udayasoorian C, Kumar R V and Jayabalakrishnan M 2011 J. Nanomater. Biostruct. 6 537

  24. Visconti A, Minervini F, Lucivera G and Gambatesa V 1991 Mycopathology 113 181

  25. Ingle A, Gade A, Pierrat S, Sonnichsen C and Rai M 2008 Curr. Nanosci. 4 141

  26. Mock J J, Barbic M, Smith D R, Shultz D A and Shultz S 2002 J. Chem. Phys. 116 6755

  27. Prabhu S and Poulose E K 2012 Nano Lett. 2 1

  28. Kaler A, Jain S and Banerjee U C 2013 BioMed. Res. Int. 2013 8

  29. Shankar S, Ahmad A and Sastry M 2003 Biotechnol. Prog. 19 1627

  30. Venkata Subbaiah K P and Savithramma N 2013 Iajpr 3 1285

  31. Sondi I and Salopek-Sondi B 2004 J. Colloid Interface Sci. 275 177

  32. Morones J R, Elechiguerra J L, Camacho A, Holt K, Kouri J B, Ramirez J T and Yacaman M J 2005 J. Nanotechnol. 16 2346

Download references

Acknowledgements

We are thankful to the Director, and Joint Director, DRDO-BU-CLS for providing necessary facilities to conduct the studies.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Nanoscience and Technology, Bharathiar University, Coimbatore, 641 046, India

    PARAMASIVAM PREMASUDHA

  2. DRDO-BU CLS, Bharathiar University Campus, Coimbatore, 641 046, India

    MUDILI VENKATARAMANA & KADIRVELU KRISHNA

  3. Department of Microbiology, PSG College of Arts and Science, Coimbatore, 641 014, India

    MARRIAPPAN ABIRAMI & RAMASAMY RAJENDRAN

  4. Department of Microbiology, Maharaja Co-education College, Perundurai, 638 052, India

    PERIYASAMY VANATHI

Authors
  1. PARAMASIVAM PREMASUDHA
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. MUDILI VENKATARAMANA
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. MARRIAPPAN ABIRAMI
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. PERIYASAMY VANATHI
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. KADIRVELU KRISHNA
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. RAMASAMY RAJENDRAN
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to MUDILI VENKATARAMANA.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

PREMASUDHA, P., VENKATARAMANA, M., ABIRAMI, M. et al. Biological synthesis and characterization of silver nanoparticles using Eclipta alba leaf extract and evaluation of its cytotoxic and antimicrobial potential. Bull Mater Sci 38, 965–973 (2015). https://doi.org/10.1007/s12034-015-0945-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12034-015-0945-5

Keywords

Search

Navigation