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Application of CRISPR-Mediated Gene Editing for Crop Improvement

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Abstract

Plant gene editing has become an important molecular tool to revolutionize modern breeding of crops. Over the past years, remarkable advancement has been made in developing robust and efficient editing methods for plants. Despite a variety of available genome editing methods, the discovery of most recent system of clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins (CRISPR-Cas) has been one of the biggest advancement in this path, with being the most efficient approach for genome manipulation. Until recently, genetic manipulations were confined to methods, like Agrobacterium-mediated transformations, zinc-finger nucleases, and TAL effector nucleases. However this technology supersedes all other methods for genetic modification. This RNA-guided CRISPR-Cas system is being rapidly developed with enhanced functionalities for better use and greater possibilities in biological research. In this review, we discuss and sum up the application of this simple yet powerful tool of CRISPR-Cas system for crop improvement with recent advancement in this technology.

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Abbreviations

CRISPR:

Clustered regularly interspaced palindromic repeats

TALEN:

Transcription activator-like effector nucleases

ZFN:

Zinc-finger nucleases

NHEJ:

Non-homologous end joining

HE:

Homing endonuclease

DSB:

Double-strand break

CRD:

Central repeat domain

RVD:

Repeat variable di-residue

PAM:

Protospacer adjacent motif

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Acknowledgements

The authors acknowledge Dr. Khem Singh Gill Akal College of Agriculture, Eternal University for providing required infrastructure and research facilities.

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  1. Department of Genetics-Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh, 173101, India

    Chandranandani Negi, Neeraj Kumar Vasistha, Pritesh Vyas & H. S. Dhaliwal

  2. Government Model College, Jhabua, Madhya Pradesh, 457661, India

    Dharmendra Singh

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  1. Chandranandani Negi
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Negi, C., Vasistha, N.K., Singh, D. et al. Application of CRISPR-Mediated Gene Editing for Crop Improvement. Mol Biotechnol 64, 1198–1217 (2022). https://doi.org/10.1007/s12033-022-00507-y

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