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Anatomical and Physiological Differences and Differentially Expressed Genes Between the Green and Yellow Leaf Tissue in a Variegated Chrysanthemum Variety

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Abstract

The leaves of the chrysanthemum variety ‘NAU04-1-31-1’ are variegated with distinct green and yellow sectors. The chlorophyll content of the yellow leaf tissue is less than that in the green one. The chloroplasts in the yellow leaf tissue were vacuolated, lacked thylakoid membrane structure and contained clusters of plastoglobuli with few or no starch grains. The yellow leaf tissue was more sensitive to photo-inhibition than the green leaf tissue. Suppression subtractive hybridization (SSHs) libraries were constructed to identify genes which were differentially transcribed in the two tissue types. The sequencing of 339 SSH clones identified 150 unigenes (93 singletons and 57 contigs), of which 85 were differentially transcribed in the green leaf tissue and 65 in the yellow leaf tissue. Unigenes associated with photosynthesis were particularly frequent, and many of these genes were up-regulated in the yellow leaf tissue. Both CmChlH which encodes the large subunit of Mg-chelatase and CmFtsH (ATP-dependent metalloprotease) were up-regulated in the yellow leaf tissue, and their transcription was regulated by light.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30872064, 31071820, 31071825), the Program for New Century Excellent Talents in University of Chinese Ministry of Education (Grant No. NCET-10-0492), Non-profit Industry Financial Program of the Ministry of Science and Technology of the People’s Republic of China (200903020) and the Fundamental Research Funds for the Central Universities (KYJ 200907, KYZ201112).

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Qingshan Chang, Sumei Chen, Yu Chen, Yanming Deng, Fadi Chen & Fei Zhang

  2. College of Forestry, Henan University of Science and Technology, Luoyang, 471003, Henan, China

    Qingshan Chang

  3. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China

    Yanming Deng

  4. Shanghai Oe Biotech Co., Ltd, No. 1, Cailun Road 720, ZhangJiang Area, Shanghai, 201210, China

    Shuwei Wang

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  1. Qingshan Chang
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Correspondence to Fadi Chen.

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Qingshan Chang and Sumei Chen contributed equally to this work reported here.

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Chang, Q., Chen, S., Chen, Y. et al. Anatomical and Physiological Differences and Differentially Expressed Genes Between the Green and Yellow Leaf Tissue in a Variegated Chrysanthemum Variety. Mol Biotechnol 54, 393–411 (2013). https://doi.org/10.1007/s12033-012-9578-8

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