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The effects of silencing of PI3K p85α on 5-FU-induced colorectal cancer cells apoptosis

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Abstract

Colorectal cancer is the third most common malignancy worldwide. 5-fluorouracil (5-FU) is the commonly used chemotherapeutic agent, however, more patients develop resistance. Phosphatidylinositol 3-kinases (PI3Ks) play a crucial role in a wide range of cellular processes associated with malignant behavior including cell growth, migration, and survival. In this study, we show increased expression of PI3K p85α during the progression of colorectal cancer. Silencing of PI3K p85α in colorectal cancer cells increased disruption of mitochondrial membrane potential and enhanced 5-FU-induced apoptosis. Furthermore, PI3K p85α-depletion results in activated expression of apoptosis-associated genes Bcl-6, Bim, and Bax. Our results suggest that knockdown of PI3K p85α is a potential therapeutic strategy in the treatment of colorectal cancer.

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Acknowledgments

This study was supported by Science and Information Technology Foundation of Guangzhou (2011J4100051) and Natural Science Foundation of Guangdong Province (S2012040006707).

Conflict of interest

We confirm that this manuscript has not been published elsewhere and is not under consideration by another journal. All authors have approved the manuscript and agree with submission to Medical Oncology. The authors have no conflict of interests to declare.

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Authors and Affiliations

  1. Gastroenterology Department, The Third Affiliated Hospital of Guangzhou Medical University, Guang Zhou, 510150, China

    Yan Sun

  2. Gastroenterology Department, Houjie Hospital Affiliated to Guangdong Medical College, Dongguan, 523900, China

    Hua Tian

  3. Oncology Department, Guang Zhou Red Cross Hospital, Guang Zhou, 510150, China

    Lin Wang

  4. Neurology Department, Guangdong 999 Brain Hospital, Guang Zhou, 510510, China

    Hui Yang

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  1. Yan Sun
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  2. Hua Tian
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Correspondence to Yan Sun.

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Sun, Y., Tian, H., Wang, L. et al. The effects of silencing of PI3K p85α on 5-FU-induced colorectal cancer cells apoptosis. Med Oncol 30, 704 (2013). https://doi.org/10.1007/s12032-013-0704-7

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  • DOI: https://doi.org/10.1007/s12032-013-0704-7

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