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Overexpression of MAP-2 via Formation of Microtubules Plays an Important Role in the Sprouting of Mossy Fibers in Epileptic Rats

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Abstract

This study aims to investigate the changes of microtubule-associated protein 2 (MAP-2) in epileptic dentate gyrus and significance in epilepsy. Alterations in MAP-2 immunoreactivity (MAP2-IR) in kindling model of epilepsy were investigated in rats with survival times ranging from 3 to 30 days. Nissl staining and electron microscopic studies were used to explore more deeply. Electron microscopic was employed to detect tubulin formation and assembly. Immunohistochemical assay was used to examine co-localization of tubulin and MMP-2. Results indicated that MAP2-IR obviously enhanced in dendrites of granule cells 3 days after kindling and remained enhanced up to 14 days (P < 0.01). It returned to control patterns 20 to 30 days after kindling (P < 0.01). Nissl staining demonstrated a negative correlation between enhancement of MAP-2 and degeneration of hilar neurons (r = −0.9274). Electron microscopic studies showed that microtubules of dendrite in dentate molecular increased 3 days after kindling and obviously increased 14 days after kindling (P < 0.05). Tubulin protein showed a well co-localization with MMP-2 protein. Treatment of paclitaxel significantly decreased tubulin formation and assembly compared with untreated group (P < 0.01). Sprouting of dentate granule cell axons and synaptic contacts between nerve terminal and dendrite were discovered. Overexpression of MAP-2 via formation of microtubules plays an important role in the sprouting of mossy fibers in epileptic rats.

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China

    Yu Li

  2. Department of Pathophysiology, Chongqing Medical University, Chongqing, 400016, China

    Li Tang

  3. Institute of Neuroscience, Chongqing Medical University, Chongqing, 400016, China

    Li Tang & Yu Li

  4. Experimental and Teaching Center, Chongqing Medical University, Chongqing, 400016, China

    Weiping Zheng

  5. Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

    Yang Lu

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  1. Li Tang
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  2. Yang Lu
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  4. Yu Li
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Correspondence to Yu Li.

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Tang, L., Lu, Y., Zheng, W. et al. Overexpression of MAP-2 via Formation of Microtubules Plays an Important Role in the Sprouting of Mossy Fibers in Epileptic Rats. J Mol Neurosci 53, 103–108 (2014). https://doi.org/10.1007/s12031-013-0204-4

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  • DOI: https://doi.org/10.1007/s12031-013-0204-4

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