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Nesfatin-1 Decreases Excitability of Dopaminergic Neurons in the Substantia Nigra

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Abstract

Nesfatin-1, a newly discovered satiety molecule which reduces feeding behavior, has been recognized as a unique regulatory neuropeptide with its multiple roles, both central and peripheral. However, whether it had neuronal modulation effect on dopaminergic neurons is largely unknown. In the present study, using whole-cell patch clamp under current-clamp mode, we investigate the effects of nesfatin-1 on the electrical activity of rat nigral dopaminergic neurons. Nesfatin-1 could produce a resting membrane potential hyperpolarization on the majority of dopaminergic neurons tested. The spike frequency decreased by 23.13 ± 5.93 and 43.20 ± 5.56 % in 5-nM and 10-nM nesfatin-1 groups, respectively. These effects persisted in the presence of ionotropic glutamate and GABA receptor antagonists. Our study suggests that nesfatin-1 postsynaptically inhibits the electrical activity of nigral dopaminergic neurons.

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Acknowledgments

This study was funded by grants from the 973 Program (2012CB526700, 2011CB504102), National Natural Science Foundation of China (81171207, 31200819), and Ministry of Education of China (20123706120002).

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Authors and Affiliations

  1. Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Medical College of Qingdao University, Qingdao, 266071, China

    Limin Shi, Haoyun Zhang, Zibin Tian, Junxia Xie & Hong Jiang

  2. Department of Pharmacology, Medical College of Qingdao University, Qingdao, 266021, China

    Chen Li & Fang Zhang

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  1. Chen Li
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  2. Fang Zhang
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  3. Limin Shi
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  4. Haoyun Zhang
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  5. Zibin Tian
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  6. Junxia Xie
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  7. Hong Jiang
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Corresponding author

Correspondence to Hong Jiang.

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Chen Li and Fang Zhang contributed equally to this work.

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Li, C., Zhang, F., Shi, L. et al. Nesfatin-1 Decreases Excitability of Dopaminergic Neurons in the Substantia Nigra. J Mol Neurosci 52, 419–424 (2014). https://doi.org/10.1007/s12031-013-0169-3

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  • DOI: https://doi.org/10.1007/s12031-013-0169-3

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