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Oxidative stress in adult growth hormone deficiency: different plasma antioxidant patterns in comparison with metabolic syndrome

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Abstract

Background and aims

Growth hormone deficiency (GHD) is a condition associated with increased cardiovascular risk and insulin-resistance. Oxidative stress (OS) could be a mechanism underlying both these phenomena. In order to investigate plasma antioxidant defenses in such condition, we evaluated adults with GHD, compared with controls and metabolic syndrome patients (MetS), studying plasma total antioxidant capacity (TAC) and coenzyme Q10 (CoQ10, lipophilic antioxidant) levels, both in its oxidized and reduced forms, correlating this data with metabolic and hormonal pattern.

Materials and methods

In this case-control study, 51 GHD, 36 controls, and 35 MetS were enrolled. An evaluation of hormonal and metabolic parameters was performed. TAC was measured using the system metmyoglobin -H202 and the chromogen ABTS, whose radical form is spectroscopically revealed; latency time (LAG) in the appearance of ABTS● is proportional to antioxidant in sample. CoQ10 was assayed by electrochemical method.

Results

Despite HOMA index was higher in both GHD and MetS (2.2 ± 0.3 and 3.1 ± 0.3 vs. 1.2 ± 0.2 in controls), only in MetS we observed lower LAG levels (64.5 ± 3.1 s vs. 82.8 ± 5.8 in GHD and 80.6 ± 6.6 in controls), suggesting an increased consumption of antioxidants. LAG significantly correlated with uric acid only in MetS (r 2 = 0.65, p < 0.001), suggesting a different pattern of antioxidants. CoQ10 exhibited a trend toward lower levels in GHD, although not significant.

Conclusions

Our data indicate that GHD, although sharing with MetS various metabolic features, including increased HOMA levels, showed a different pattern of plasma antioxidants, suggesting inadequate reactivity toward radical production rather than an antioxidants consumption as in MetS.

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Acknowledgements

We would like to thank Mr. Primiano Palma for his skillful assistance.

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Authors and Affiliations

  1. Operative Unit of Endocrinology, Catholic University of the Sacred Heart, Largo A. Gemelli 8, Rome, 00168, Italy

    Antonio Mancini, Chantal Di Segni, Carmine Bruno, Giulio Olivieri & Alfredo Pontecorvi

  2. Department of Obstetrics and Gynecology, Catholic University of the Sacred Heart, Largo A. Gemelli 8, Rome, 00168, Italy

    Francesco Guidi

  3. Institute of Biochemistry and Clinical Biochemistry, Catholic University of the Sacred Heart, Largo F. Vito 1, Rome, 00168, Italy

    Andrea Silvestrini & Elisabetta Meucci

  4. Department of Life and Environmental Sciences, Polytechnic University of Marche, Via Brecce Bianche, Ancona, 60131, Italy

    Patrick Orlando, Sonia Silvestri & Luca Tiano

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  1. Antonio Mancini
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Mancini, A., Di Segni, C., Bruno, C. et al. Oxidative stress in adult growth hormone deficiency: different plasma antioxidant patterns in comparison with metabolic syndrome. Endocrine 59, 130–136 (2018). https://doi.org/10.1007/s12020-017-1468-1

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