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Expression and regulation of adipocyte fatty acid binding protein in granulosa cells and its relation with clinical characteristics of polycystic ovary syndrome

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Abstract

Increased expression of adipocyte fatty acid binding protein (FABP4) is associated with type 2 diabetic, high triglycerides, increased lipid peroxidation, and inflammation markers. To study the expression of FABP4 mRNA in granulosa cells of patients with polycystic ovary syndrome (PCOS) and the impact of testosterone, insulin, and PPARγ agonist rosiglitazone on granulosa cells (GCs), and to investigate the relationship of serum FABP4 levels with clinical characteristics in patients with PCOS. The expression of FABP4 mRNA in GCs of patients with PCOS and normal controls were assayed by RT-PCR. We assessed the level of FABP4 mRNA after treatment with testosterone, insulin, and rosiglitazone in GCs from normal controls. Serum FABP4 were assayed from 96 patients with PCOS (obese and nonobese 48 cases, respectively) and 80 healthy normal controls (obese and the nonobese 40 cases, respectively). The expression of FABP4 mRNA was higher in the GCs of PCOS than that of the controls (P  < 0.05). FABP4 mRNA expression was up-regulated by testosterone, insulin, and rosiglitazone at different dosages. Serum FABP4 levels were higher in the nonobese PCOS group than that of the nonobese controls (8.9 ± 5.1 ng/ml vs. 4.8 ± 0.7 ng/ml), and in the obese PCOS group than that of the obese controls (28.2 ± 14.0 ng/ml vs. 15.6 ± 6.6 ng/ml), respectively (P < 0.05). Multiple linear regression analyses showed that serum FABP4 level was independently associated with HOMA-IR, BMI, and testosterone (P < 0.05). Increased FABP4 was related to the clinical characteristics of PCOS.

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References

  1. E.S. Knochenhauer, T.J. Key, M. Kahsar-Miller, W. Waggoner, L.R. Boots, R. Azziz, J. Clin. Endocrinol. Metab. 83, 3078 (1998)

    Article  PubMed  CAS  Google Scholar 

  2. D.S. Guzick, Obstet. Gynecol. 103, 181 (2004)

    Article  PubMed  CAS  Google Scholar 

  3. S. Franks, N. Engl. J. Med. 333, 853 (1995)

    Article  PubMed  CAS  Google Scholar 

  4. A. Dunaif, Endocr. Rev. 18, 774 (1997)

    Article  PubMed  CAS  Google Scholar 

  5. T.M. Barber, M.I. McCarthy, J.A. Wass, S. Franks, Clin. Endocrinol. (Oxf) 65, 137 (2006)

    Article  CAS  Google Scholar 

  6. C.N. Wijeyaratne, A.H. Balen, J.H. Barth, P.E. Belchetz, Clin. Endocrinol. (Oxf) 57, 343 (2002)

    Article  CAS  Google Scholar 

  7. D.A. Ehrmann, D.R. Liljenquist, K. Kasza, R. Azziz, R.S. Legro, M.N. Ghazzi, J. Clin. Endocrinol. Metab. 91, 48 (2006)

    Article  PubMed  CAS  Google Scholar 

  8. S. Wild, T. Pierpoint, P. McKeigue, H. Jacobs, Clin. Endocrinol. (Oxf). 52, 595 (2000)

    Article  CAS  Google Scholar 

  9. C.C. Kelly, H. Lyall, J.R. Petrie, G.W. Gould, J.M. Connell, N. Sattar, J. Clin. Endocrinol. Metab. 86, 2453 (2001)

    Article  PubMed  CAS  Google Scholar 

  10. N. Boulman, Y. Levy, R. Leiba, S. Shachar, R. Linn, O. Zinder, Z. Blumenfeld, J. Clin. Endocrinol. Metab. 89, 2160 (2004)

    Article  PubMed  CAS  Google Scholar 

  11. G.S. Hotamisligil, R.S. Johnson, R.J. Distel, R. Ellis, V.E. Papaioannou, B.M. Spiegelman, Science 274, 1377 (1996)

    Article  PubMed  CAS  Google Scholar 

  12. K.T. Uysal, L. Scheja, S.M. Wiesbrock, Endocrinology 141, 3388 (2000)

    Article  PubMed  CAS  Google Scholar 

  13. G. Tuncman, E. Erbay, X. Hom, I. De Vivo, H. Campos, E.B. Rimm, G.S. Hotamisligil, Proc. Natl. Acad. Sci. USA 103, 6970 (2006)

    Article  PubMed  CAS  Google Scholar 

  14. A. Xu, Y. Wang, J.Y. Xu, D. Stejskal, S. Tam, J. Zhang, N.M. Wat, W.K. Wong, K.S. Lam, Clin. Chem. 52, 405 (2006)

    Article  PubMed  CAS  Google Scholar 

  15. A. Xu, A.W. Tso, B.M. Cheung, Y. Wang, N.M. Wat, C.H. Fong, D.C. Yeung, E.D. Janus, P.C. Sham, K.S. Lam, Circulation 115, 1537 (2007)

    Article  PubMed  CAS  Google Scholar 

  16. A.W. Tso, A. Xu, P.C. Sham, N.M. Wat, Y. Wang, C.H. Fong, B.M. Cheung, E.D. Janus, K.S. Lam, Care 30, 2667 (2007)

    CAS  Google Scholar 

  17. J.B. Boord, S. Fazio, M.F. Linton, Curr. Opin. Lipidol. 13, 141 (2002)

    Article  PubMed  CAS  Google Scholar 

  18. Z.X. Hu, J. Qiao, M.Z. Li, X.W. Zhang, Y.J. Chen, R. Li, C.L. Sun, Beijing. Da. Xue. Xue. Bao. 36, 600 (2004)

    PubMed  CAS  Google Scholar 

  19. The Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group, Fertil. Steril. 81, 19 (2004)

    Google Scholar 

  20. L. Poretsky, D. Seto-Young, A. Shrestha, S. Dhillon, N. Mirjany, H.-C. Liu, M.C. Yih, Z. Rosenwaks, J. Clin. Endocrinol. Metab. 86, 3115 (2001)

    Article  PubMed  CAS  Google Scholar 

  21. L. Makowski, G.S. Hotamisligil, J. Nutr. 134, 2464S (2004)

    PubMed  CAS  Google Scholar 

  22. M.R. Nourani, Y. Owada, N. Kitanaka, H. Sakagami, H. Hoshi, H. Iwasa, F. Spener, H. Kondo, J. Mol. Histol. 36, 491 (2005)

    Article  PubMed  CAS  Google Scholar 

  23. J.B. Boord, K. Maeda, L. Makowski, V.R. Babaev, S. Fazio, M.F. Linton, G.S. Hotamisligil, Circulation 110, 1492 (2004)

    Article  PubMed  CAS  Google Scholar 

  24. R.J. Perera, E.G. Marcusson, S. Koo, X. Kang, Y. Kim, N. White, N.M. Dean, Gene 369, 90 (2006)

    Article  PubMed  CAS  Google Scholar 

  25. N.S. Tan, N.S. Shaw, N. Vinckenbosch, P. Liu, R. Yasmin, B. Desvergne, W. Wahli, N. Noy, Mol. Cell. Biol. 22, 5114 (2002)

    Article  PubMed  CAS  Google Scholar 

  26. C. Schachtrup, T. Emmler, B. Bleck, A. Sandqvist, F. Spener, Biochem. J. 382, 239 (2004)

    Article  PubMed  CAS  Google Scholar 

  27. N. Tinfo, C. Komar, Reproduction 133, 187 (2007)

    Article  PubMed  CAS  Google Scholar 

  28. C.E. Minge, B.D. Bennett, R.J. Norman, R.L. Robker, Endocrinology 149, 2646 (2008)

    Article  PubMed  CAS  Google Scholar 

  29. D. Stejskal, M. Karpisek, Eur. J. Clin. Invest. 36, 621 (2006)

    Article  PubMed  CAS  Google Scholar 

  30. A. Cabré, I. Lázaro, J. Girona, J.M. Manzanares, F. Marimón, N. Plana, M. Heras, L. Masana, Atherosclerosis 195, e150 (2007)

    Article  PubMed  Google Scholar 

  31. D.C. Yeung, A. Xu, C.W. Cheung, N.M. Wat, M.H. Yau, C.H. Fong, M.Y. Chau, K.S. Lam, Arterioscler. Thromb. Vasc. Biol. 27, 1796 (2007)

    Article  PubMed  CAS  Google Scholar 

  32. M. Möhlig, M.O. Weickert, E. Ghadamgadai, A. Machlitt, B. Pfüller, A.M. Arafat, A.F. Pfeiffer, C. Schöfl, Eur. J. Endocrinol. 157, 195 (2007)

    Article  PubMed  Google Scholar 

  33. R. Pasquali, F. Casimirri, Clin. Endocrinol. (Oxf). 39, 1 (1993)

    Article  CAS  Google Scholar 

  34. L. Morin-Papunen, K. Rautio, A. Ruokonen, P. Hedberg, M. Puukka, J.S. Tapanainen, J. Clin. Endocrinol. Metab. 88, 4649 (2003)

    Article  PubMed  CAS  Google Scholar 

  35. A. Dunaif, K.R. Segal, W. Futterweit, A. Dobrjansky, Diabetes 38, 1165 (1989)

    Article  PubMed  CAS  Google Scholar 

  36. W.H. Hu, J. Qiao, R. Li, L.N. Wang, Zhonghua.Yi. Xue. Za. Zhi. 86, 3186 (2006)

    PubMed  CAS  Google Scholar 

  37. Y. Fu, N. Luo, M.F. Lopes-Virella, W.T. Garvey, Atherosclerosis 165, 259 (2002)

    Article  PubMed  CAS  Google Scholar 

  38. L. Makowski, K.C. Brittingham, J.M. Reynolds, J. Suttles, G.S. Hotamisligil, J. Biol. Chem. 280, 12888 (2005)

    Article  PubMed  CAS  Google Scholar 

  39. L. Scheja, L. Makowski, K.T. Uysal, S.M. Wiesbrock, D.R. Shimshek, D.S. Meyers, M. Morgan, R.A. Parker, G.S. Hotamisligil, Diabetes 48, 1987 (1999)

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from National Natural Science Foundation (National Science Fund for Distinguished Young Scholars) 30825038: Gynecologic Endocrinology: Study on Polycystic Ovary Syndrome through Metabolic. We also thank Dr. Huai L. Feng (Center for Human Reproduction, North Shore University Hospital, NYU School of Medicine) for critical reading of the manuscript.

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All authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Peking University Third Hospital, Beijing, 100083, China

    Weihong Hu & Jie Qiao

  2. Department of Gynecology and Obstetrics, General Hospital of Chinese People’s Armed Police Forces, Beijing, 100039, China

    Weihong Hu

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  1. Weihong Hu
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  2. Jie Qiao
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Correspondence to Jie Qiao.

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Hu, W., Qiao, J. Expression and regulation of adipocyte fatty acid binding protein in granulosa cells and its relation with clinical characteristics of polycystic ovary syndrome. Endocrine 40, 196–202 (2011). https://doi.org/10.1007/s12020-011-9495-9

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  • DOI: https://doi.org/10.1007/s12020-011-9495-9

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