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Developmental exposure to pentachlorophenol affects the expression of thyroid hormone receptor β1 and synapsin I in brain, resulting in thyroid function vulnerability in rats

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Abstract

Pentachlorophenol (PCP), a component of biocides and a contaminant in diverse tissue samples from humans from various geographic areas, disrupts regulatory effects of thyroid hormones. Here we examined the effects of developmental exposure of rats to PCP on various aspects of brain development, male reproductive function, and adrenal function, all of which are under thyroid hormones regulation. PCP was administered to dams and their offspring via drinking water (6.6 mg l−1) during gestation and lactation. Tissue samples were obtained from dams, 3-week-old weanling pups, and 12-week-old pups. Gene expressions of thyroid hormone receptor β1 and synapsin I, factors that promote brain growth, was increased in the cerebral cortex of PCP-treated weanling females, whereas plasma concentrations of total thyroxine were decreased in dams and weanling pups, and plasma thyroid-stimulating hormone concentrations were higher in PCP-treated weanling males. PCP caused a decrease in plasma corticosterone concentrations in 12-week-old female rats, but not in male rats or weanling females. PCP-treated male pups had significantly increased testis weight at 12 week of age. No overt signs of toxicity were noted throughout this study. Our results show that PCP exposure during development causes thyroid function vulnerability, testicular hypertrophy in adults, and aberrations of brain gene expression.

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Acknowledgments

We thank Dr. G.D. Niswender (Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO, USA) for the generous gift of antisera to testosterone and estradiol. We thank Yukiko Okada (National Institute for Environmental Studies) for her experimental assistance. This work was supported in part by the “High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT (to T.H.), and a Grant-in-Aid for Scientific Research (B-1831004) from the Japan Society for the Promotion of Science (to K.T.). Kawaguchi received a fellowship from the Japan Society for the Promotion of Science.

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  1. Kaori Morohoshi

    Present address: Yokohama Laboratory, Mitsubishi Chemical Safety Institute Ltd, 1000 Kamoshida-cho, Aoba-ku, Yokohama-shi, Kanagawa, 227-0033, Japan

Authors and Affiliations

  1. Faculty of Pharmacy and Research Institute of Pharmaceutical Science, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo, 202-8585, Japan

    Maiko Kawaguchi & Toshiyuki Himi

  2. Environmental Health Sciences Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba-shi, Ibaraki, 305-8506, Japan

    Kaori Morohoshi, Rie Yanagisawa & Hirohisa Takano

  3. Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan

    Erina Saita, Gen Watanabe & Kazuyoshi Taya

  4. Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu, 501-1193, Japan

    Erina Saita, Gen Watanabe & Kazuyoshi Taya

  5. Research Center for Environmental Risk, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba-shi, Ibaraki, 305-8506, Japan

    Masatoshi Morita

  6. Department of Bioresources, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Masatoshi Morita

  7. Division of Environmental Health Sciences, Department of Social Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake Town, Miyazaki, 889-1692, Japan

    Hideki Imai

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  1. Maiko Kawaguchi
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Correspondence to Kazuyoshi Taya.

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Kawaguchi, M., Morohoshi, K., Saita, E. et al. Developmental exposure to pentachlorophenol affects the expression of thyroid hormone receptor β1 and synapsin I in brain, resulting in thyroid function vulnerability in rats. Endocr 33, 277–284 (2008). https://doi.org/10.1007/s12020-008-9086-6

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