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Pectin Oligosaccharides Enhance α2,6-Sialylation Modification that Promotes Apoptosis of Bladder Cancer Cells by Targeting the Hedgehog Pathway

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Abstract

Although pectin oligosaccharide (POS) can inhibit the growth and proliferation of gastric, colon, prostate, breast, melanoma, and leukemia cells, its effect on bladder cancer remains unknown. Therefore, screening and identification of factors associated with the sensitivity of bladder cancer to drugs and elucidation of their molecular mechanisms will help provide a theoretical basis for establishing postoperative systemic chemotherapy for patients with bladder cancer. We showed that POS promoted the apoptosis of bladder cancer cells, and this finding was consistent with enhanced α2,6-sialylation post-modification. Moreover, POS activated the Hedgehog pathway, the inhibition of which regulated the tumorigenicity of bladder cancer cells in vivo. These findings were consistent with our results in vitro. We conclude that POS promotes the apoptosis of bladder cancer and offers new insights and evidence for the development of individualized treatment strategies. Schema of molecular events underlying POS-induced inhibition of bladder cancer cell proliferation.

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Data Availability

Data are included in the article and in supplementary information.

Abbreviations

IHC:

immunohistochemical analysis

POS:

pectin oligosaccharide

SA:

sialic acid

ST:

sialyltransferase

ST6Gal-1:

β-galactoside α2,6-sialyltransferase 1

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Acknowledgements

This study was approved by Research Project of Liaoning Provincial Department of Education (No. JYTQN2020037).

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Authors and Affiliations

  1. Department of Hepatobiliary, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, China

    Yinpeng Huang

  2. Physical Examination Centre, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, China

    Tianyi Wang

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  1. Yinpeng Huang
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  2. Tianyi Wang
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Correspondence to Tianyi Wang.

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The authors declare no competing interests.

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All animal care and experiments proceeded in accordance with our Institutional Animal Care protocols. All animal experiments complied with the guidelines outlined by the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

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Huang, Y., Wang, T. Pectin Oligosaccharides Enhance α2,6-Sialylation Modification that Promotes Apoptosis of Bladder Cancer Cells by Targeting the Hedgehog Pathway. Cell Biochem Biophys 79, 719–728 (2021). https://doi.org/10.1007/s12013-021-00996-9

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