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Identification of the microRNA Expression Profile in the Regenerative Neonatal Mouse Heart by Deep Sequencing

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Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that are involved in key biological processes, including development, differentiation, and regeneration. The global miRNA expression profile that regulates the regenerative potential of the neonatal mouse heart has not been reported. We performed deep sequencing to determine the genome-wide miRNA expression profile of the neonatal mouse heart at three key ages (1, 6, and 7 days). The miRNAs at least 1.4-fold differentially expressed between the three time points were selected for further analysis. Two miRNAs (mmu-miR-22-5p and mmu-miR-338-3p) were significantly upregulated, and nine miRNAs (mmu-miR-324-5p, mmu-miR-337-5p, mmu-miR-339-5p, mmu-miR-365-1-5p, mmu-miR-500-3p, mmu-miR-505-5p, mmu-miR-542-5p, mmu-miR-668-3p, and mmu-miR-92a-1-5p) were significantly downregulated in cardiac tissue of 7-day-old mice compared to 1- and 6-day-old mice. The expression patterns of five significantly different miRNAs were verified by quantitative real-time PCR. Furthermore, the potential targets of these putative miRNAs were suggested using miRNA target prediction tools. The candidate target genes are involved in the myocardial regenerative process, with a prominent role for the Notch signaling pathway. Our study provides a valuable resource for future investigation of the biological function of miRNAs in heart regeneration.

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Acknowledgments

This study was supported by Grants from the National Natural Science Foundation of China (Nos. 81070138 and 81200126) and the National Natural Science Foundation of Jiangsu Province of China (No. BK2010582).

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Authors and Affiliations

  1. Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People’s Republic of China

    H. L. Liu & L. M. Qian

  2. State Key Laboratory of Reproductive Medicine, Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, 210004, People’s Republic of China

    J. G. Zhu & C. Zhu

  3. Medical Intensive Care Unit, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing, 210004, People’s Republic of China

    Y. Q. Liu

  4. Department of Pediatrics, Maanshan Municipal Hospital Group, Maanshan, 243000, People’s Republic of China

    Z. G. Fan

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  1. H. L. Liu
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  2. J. G. Zhu
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  3. Y. Q. Liu
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  4. Z. G. Fan
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  5. C. Zhu
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  6. L. M. Qian
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Correspondence to C. Zhu or L. M. Qian.

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Liu HL and Zhu JG contributed equally to this work.

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Liu, H.L., Zhu, J.G., Liu, Y.Q. et al. Identification of the microRNA Expression Profile in the Regenerative Neonatal Mouse Heart by Deep Sequencing. Cell Biochem Biophys 70, 635–642 (2014). https://doi.org/10.1007/s12013-014-9967-7

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  • DOI: https://doi.org/10.1007/s12013-014-9967-7

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