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Comparative Analysis of Differentially Expressed Genes in Chondrocytes from Rats Exposed to Low Selenium and T-2 Toxin

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Abstract

The aim of this study was to construct rat models of environmental risk factors for Kashin-Beck disease (KBD) with low selenium and T-2 toxin levels and to screen the differentially expressed genes (DEGs) between the rat models exposed to environmental risk factors. The Se-deficient (SD) group and T-2 toxin exposure (T-2) group were constructed. Knee joint samples were stained with hematoxylin–eosin, and cartilage tissue damage was observed. Illumina high-throughput sequencing technology was used to detect the gene expression profiles of the rat models in each group. Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis were performed and five differential gene expression results were verified by quantitative real-time polymerase chain reaction (qRT‒PCR). A total of 124 DEGs were identified from the SD group, including 56 upregulated genes and 68 downregulated genes. A total of 135 DEGs were identified in the T-2 group, including 68 upregulated genes and 67 downregulated genes. The DEGs were significantly enriched in 4 KEGG pathways in the SD group and 9 KEGG pathways in the T-2 group. The expression levels of Dbp, Pc, Selenow, Rpl30, and Mt2A were consistent with the results of transcriptome sequencing by qRT‒PCR. The results of this study confirmed that there were some differences in DEGs between the SD group and the T-2 group and provided new evidence for further exploration of the etiology and pathogenesis of KBD.

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Data Availability

All data generated or used during the study are available from the corresponding author and first author upon reasonable request.

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Funding

This study was financially supported by the National Natural Science Foundation of China (82273752 and 81620108026), Natural Science Basic Research Plan in Shaanxi Province of China (2023-JC-YB-704), the China Postdoctoral Science Foundation (2022M712526, 2021M692543 and 2022M722523), the Shaanxi Postdoctoral Foundation (2018BSHYDZZ47 and 2018BSHEDZZ96) and the Key R&D Program of Shaanxi Province (NO. 2022SF-361). We thank laboratory technician Zengtie Zhang for expert experimental advice.

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Author notes

  1. Yifan Wu and Yi Gong contributed equally to this study.

Authors and Affiliations

  1. Department of Occupational and Environmental Health, School of Public Health, Xi’an Jiaotong University Health Science Center, Xi’an, Shaanxi, 710061, People’s Republic of China

    Yifan Wu, Yi Gong, Yanli Liu, Feihong Chen, Ruitian Huang & Xi Wang

  2. School of Public Health, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi’an Jiaotong University Health Science Center, No.76 Yanta West Road, Xi’an, Shaanxi, 710061, People’s Republic of China

    Sijie Chen, Feiyu Zhang, Chaowei Wang, Shujin Li, Minhan Hu, Xiong Guo, Xi Wang & Yujie Ning

  3. Clinical Research Center for Endemic Disease of Shaanxi Province, The Second Affiliated Hospital of Xi’an Jiaotong University, No.157 Xi Wu Road, Xi’an, 710004, Shaanxi, People’s Republic of China

    Xiong Guo

  4. Key Laboratory for Disease Prevention and Control and Health Promotion of Shaanxi Province, Xi’an Jiaotong University, Xi’an, China

    Xi Wang

  5. School of Nursing, Health Science Center, Xi’an Jiaotong University, Xi’an, 710061, China

    Lei Yang

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  1. Yifan Wu
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Contributions

WYF, YJN, XW, and XG designed the study. YG, MHH, RTH, YLL, FHC, FYZ, SJC, SJL, LY, and CWW performed the analysis and interpretation of the data. YJN, XW, and XG analyzed the results and drafted the manuscript. All authors critically reviewed and amended the manuscript, and all authors read and approved the final manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Xi Wang or Yujie Ning.

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This study was approved by the Ethics Committee of Xi’an Jiaotong University.

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Wu, Y., Gong, Y., Liu, Y. et al. Comparative Analysis of Differentially Expressed Genes in Chondrocytes from Rats Exposed to Low Selenium and T-2 Toxin. Biol Trace Elem Res 202, 1020–1030 (2024). https://doi.org/10.1007/s12011-023-03725-w

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