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Age-Dependent Developmental Changes of Selenium Content and Selenoprotein Expression and Content in Longissimus Dorsi Muscle and Liver of Duroc Pigs

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Abstract

The trace element selenium (Se) plays a key role in development and various physiological processes, mainly through its transformation into selenoproteins. To investigate the developmental patterns of Se content and expression of selenoproteins, the liver and longissimus dorsi (LD) muscle of Duroc pigs were collected at 1, 21, 80, and 185 days of age (7 pigs each age) for the determination of Se content, mRNA expression of selenoproteins, and concentrations of glutathione peroxidase (GPX), thioredoxin reductase (TrxR or TXNRD), and selenoprotein P (SELP). The results showed that age significantly affected the expression of GPX1, GPX2, GPX3, TXNRD1, TXNRD2, TXNRD3, iodothyronine deiodinases 2 (DIO2), DIO3, SELF, SELH, SELM, SELP, SELS, SELW, and selenophosphate synthetase2 (SPS2) in the liver, as well as GPX3, GPX4, TXNRD1, TXNRD2, DIO2, DIO3, SELF, SELN, SELP, SELR, SELS, and SELW in the LD muscle of Duroc pigs. The concentrations of GPX, TrxR, and SELP showed an increasing trend with age, and they were positively correlated with Se content at 1, 21, and 185 days of age and negatively correlated at 80 days of age, both in the liver and LD muscle. The Se content decreased at the age of 80 days, especially in the LD muscle. In summary, our study revealed developmental changes in Se content and expression of selenoproteins in the liver and LD muscle of Duroc pigs at different growth stages, which provided a theoretical basis for further study of Se nutrition and functions of selenoproteins.

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The data presented in the study are included in the article. Further inquiries can be directed to the corresponding authors.

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Funding

This work was jointly supported by the Key Project of Regional Innovation and Development Joint Fund of National Natural Science Foundation of China (U20A2056) and National Students’ Platform for Innovation and Entrepreneurship Training Program (202210542036).

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Author notes

  1. Yiwen He, Peng Guan, and Yan Zeng contributed equally to this work.

Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Yiwen He, Peng Guan, Yan Zeng & Le Huang

  2. Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolism Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China

    Yiwen He, Peng Guan, Yan Zeng, Le Huang, Can Peng, Xiangfeng Kong & Xihong Zhou

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  1. Yiwen He
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Contributions

Yiwen He, Peng Guan, and Yan Zeng conceived and designed the experiments; Can Peng participated in the final interpretation of the data; Yiwen He, Peng Guan, Yan Zeng, and Le Huang acquired and analyzed the data; Yiwen He, Peng Guan, and Yan Zeng drafted the manuscript. Xihong Zhou and Xiangfeng Kong supervised the whole experiment and reviewed the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Xiangfeng Kong or Xihong Zhou.

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The study was conducted according to the principles of the Animal Care Committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences and was approved by the animal welfare committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences (Approval no. 20200018).

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He, Y., Guan, P., Zeng, Y. et al. Age-Dependent Developmental Changes of Selenium Content and Selenoprotein Expression and Content in Longissimus Dorsi Muscle and Liver of Duroc Pigs. Biol Trace Elem Res 202, 182–189 (2024). https://doi.org/10.1007/s12011-023-03674-4

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