Abstract
Liver fibrosis is a worldwide public health problem due to its life-threatening complications, including portal hypertension, liver failure, cirrhosis, and hepatocellular carcinoma (HCC). Liver fibrosis is the net result of a complex excessive accumulation of extracellular matrix (ECM). Activation of hepatic stellate cells (HSCs) are the cause of deposition of ECM and are commonly recognized as a key step in liver fibrosis. The aim of this study was to investigate the effect of foreskin-derived mesenchymal stem cells treated with boron compounds on liver fibrosis. Rats were injected intraperitoneally with thioacetamide (TAA) at a dose of 150 mg/kg except sham and control groups’ rats. Thioacetamide (TAA), foreskin-derived mesenchymal stem cells (TAA + FSDMSC), FSDMSC treated with boric acid (TAA + FSDMSC + BA), FSDMSC treated with sodium pentaborate pentahydrate (TAA + FSDMSC + NaB), control and sham groups were studied. Boron compound treated foreskin-derived mesenchymal stem cells were injected into the tail vein, and evaluations were conducted after 4 weeks and liver tissues were obtained for structural, immunohistochemical, and western blot studies and blood samples were taken for biochemical analysis. FSDMSC (BA) alleviates TAA-induced rats liver fibrosis, and BA showed a positive effect on foreskin-derived mesenchymal stem cells viability. After using BA-treated mesenchymal stem cells, we observed that there was regression in the fibrotic areas at TAA-induced liver fibrosis. The result demonstrates that the contribution of TAA + FSDMSC and TAA + FSDMSC (NaB) at the level of structure is not effective in regression of fibrosis in TAA-generated liver fibrosis. We concluded that FSDMSC treated with BA may be a factor in the regression of fibrosis.
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The data that support the findings of this study are available from the corresponding author upon request.
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Acknowledgements
We would like to thank Nezaket Turkel Sesli for her close support in cell culture studies, Ilkay Dogan who evaluated the statistical data, and Pau Sancho-Bru and Mehmet Kahraman for their valuable comments on the manuscript.
This project with the number of TPF18009 was supported by the Aydın Adnan Menderes University. Part of this study was conducted at Yeditepe University, Department of Genetics and Bioengineering, Istanbul.
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Concept and design: Alpaslan Gokcimen, Fikrettin Sahin, and Guluna Erdem Koc. Material preparation, data collection and analysis were performed by Alpaslan Gokcimen, Fikrettin Sahin, and Guluna Erdem Koc. The first draft of the manuscript was written by Guluna Erdem Koc and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ethical approval was obtained with Yeditepe University Experimental Animals Ethics Committee decision number 697 dated 14.08.2018.
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Erdem Koc, G., Gokcimen, A. & Sahin, F. The Effect of Boric Acid and Sodium Pentaborate Pentahydrate-Treated Foreskin Derived Mesenchymal Stem Cells on Liver Fibrosis. Biol Trace Elem Res 201, 4834–4849 (2023). https://doi.org/10.1007/s12011-023-03565-8
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DOI: https://doi.org/10.1007/s12011-023-03565-8