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Impact of Nickel Oxide Nanoparticles (NiO) on Oxidative Stress Biomarkers and Hemocyte Counts of Mytilus galloprovincialis

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Abstract

In this study, the toxic effects of nickel oxide nanoparticles (NiO-NPs) on the model organism Mediterranean mussel (Mytilus galloprovincialis) gill, digestive gland, and hemolymph tissues for 96 h were investigated. Lipid peroxidation (MDA) determination was performed to reveal the oxidative stress generation potential of nanoparticles, and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) enzyme levels were measured to determine antioxidant responses. Lysosomal membrane stability and total hemocyte counts were performed to determine cytotoxic effects. All parameters were altered in different concentrations of NiO-NPs (2, 20, and 200 mg L−1). The SOD levels increased depending on the concentration (p < 0.05), and the increases in CAT, GPx, and GST levels were lower at 20 mg L−1 concentration (p < 0.05). There was a slight difference between the exposure and the control groups in terms of GR enzyme. The MDA level increased in parallel with the concentration (p < 0.05), the stability of the cell membrane (p < 0.05), and the number of hemocyte cells decreased as a result of exposure (p < 0.05). The results emphasize that NiO-NPs may have negative effects on the aquatic environment.

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Data Availability

The datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.

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Acknowledgements

The author would like to thank Gelibolu Seafood Company, from which the study material was provided, and the Nanograph Nanotechnology, where nanoparticle and characterization information was provided.

Funding

This study was funded by Çanakkale Onsekiz Mart University Scientific Research Projects Commission (Project No: FHD-2021–3601).

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  1. Department of Biology, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, 17100, Canakkale, Turkey

    Selin Ertürk Gürkan

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Gürkan, S.E. Impact of Nickel Oxide Nanoparticles (NiO) on Oxidative Stress Biomarkers and Hemocyte Counts of Mytilus galloprovincialis. Biol Trace Elem Res 200, 3429–3441 (2022). https://doi.org/10.1007/s12011-022-03189-4

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