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Ecofriendly Synthesis of Biosynthesized Copper Nanoparticles with Starch-Based Nanocomposite: Antimicrobial, Antioxidant, and Anticancer Activities

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Abstract

In recent years, polysaccharides-based nanocomposites have been used for biomedical applications. In the current study, a nanocomposite based on myco-synthesized copper nanoparticles (CuNPs) and starch was prepared. The prepared nanocomposite was fully characterized using UV–visible spectroscopy (UV–vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX), mapping, transmission electron microscope (TEM), and dynamic light scattering (DLS). Results revealed that this nanocomposite is characterized by nano spherical shape ranged around 200 nm as well as doped with CuNPs with size about 9 nm. Antimicrobial, antioxidant, and anticancer activities of the prepared nanocomposite were evaluated. Results revealed that CuNPs-based nanocomposite exhibited outstanding antibacterial and antifungal activity toward Escherichia coli ATCC25922, Bacillus subtilis ATCC605, Candida albicans ATCC90028, Cryptococcus neoformance ATCC 14,116, Aspergillus niger RCMB 02,724, A. terreus RCMB 02,574, and A. fumigatus RCMB 02,568. Moreover, CuNPs-based nanocomposite has a strong antioxidant activity as compared to ascorbic acid, where IC50 was 18 µg/mL. Cytotoxicity test of CuNPs-based nanocomposite revealed that this nanocomposite is safe in use, where IC50 was 185.1 µg/mL. Furthermore, CuNPs-based nanocomposite exhibited potential anticancer activity against MCF7 cancerous cell line where IC50 was 62.8 µg/mL which was better than CuNPs alone. In conclusion, the prepared CuNPs with starch-based nanocomposite is promising for different biomedical applications as antimicrobial, antioxidant, and anticancer activities.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to acknowledge National Research Centre and Faculty of Science, Al-Azhar University, Cairo, Egypt.

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Authors and Affiliations

  1. Cellulose & Paper Department, National Research Centre, 33 El-Bohouth St. (Former El-Tahrir St.), Dokki, Giza, 12622, Egypt

    Mohamed Hasanin

  2. Biology Department, Faculty of Science, Jazan University, Jazan, Saudi Arabia

    Mohamed A. Al Abboud & Mohamed M. Alawlaqi

  3. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt

    Tarek M. Abdelghany & Amr H. Hashem

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  1. Mohamed Hasanin
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  2. Mohamed A. Al Abboud
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  4. Tarek M. Abdelghany
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  5. Amr H. Hashem
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Contributions

Conceptualization: M.H., A.H.H.; methodology: M.H., M.A.A., M.M.A., T.M.A., A.H.H.; formal analysis and investigation: M.H., M.A.A., M.M.A., T.M.A., A.H.H.; writing—original draft preparation: M.H., A.H.H.; writing—review and editing: M.H., T.M.A., A.H.H.; resources: M.H., M.A.A., M.M.A., T.M.A., A.H.H.

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Correspondence to Amr H. Hashem.

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Hasanin, M., Al Abboud, M.A., Alawlaqi, M.M. et al. Ecofriendly Synthesis of Biosynthesized Copper Nanoparticles with Starch-Based Nanocomposite: Antimicrobial, Antioxidant, and Anticancer Activities. Biol Trace Elem Res 200, 2099–2112 (2022). https://doi.org/10.1007/s12011-021-02812-0

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